J Endod:罹患慢性根尖脓肿的未发育完成前磨牙牙髓再生的疗效观察

2020-03-10 lishiting MedSci原创

目前,较少有研究通过免疫荧光观察分析感染人牙齿实施牙髓再生治疗(REPs)后的组织形成情况。这篇报道的目的是为了评估需要反复根管消毒的牙齿实施REPs后的免疫荧光组织学疗效。

目前,较少有研究通过免疫荧光观察分析感染人牙齿实施牙髓再生治疗(REPs)后的组织形成情况。这篇报道的目的是为了评估需要反复根管消毒的牙齿实施REPs后的免疫荧光组织学疗效。

11岁的女孩牙外突(#29)出现折裂伴随窦道形成。患者3次就诊完成机械和化学去感染过程,以去除临床体征和症状并达到实施REP的要求。根尖损伤在术后12个月愈合,尽管牙齿对于敏感测试依旧无反应。在术后18个月,牙齿因正畸需要拔除。采用组织学和免疫荧光技术对牙齿进行染色以观察牙本质涎磷蛋白(DSPP), 骨桥蛋白(OPN), 骨膜素和髓磷脂碱蛋白的表达情况。

结果显示,组织学检测并未观察到全新形成的牙髓牙本质复合体的形成。可见新形成的组织连接至已存在的牙本质小管中。基于其所在的位置,非成牙本质细胞样细胞表达不同比例的OPN和DSPP。挨近MTA的细胞表达DSPP和OPN,但是在根尖区域的细胞主要表达OPN。髓磷脂碱蛋白表达于新形成组织的中央并且没有扩展至冠1/3。骨膜素高表达并贯穿于整个根管,表明活跃的修复过程取代了再生。

结论:这篇研究显示了REP治疗顽固性感染的临床效果,组织学结果呈现为修复表现。在临床治疗过程中的牙髓再生可能会受到宿主反应因子之间复杂相互作用的影响。

原始出处:

Lui JN, Lim WY, et al. An Immunofluorescence Study to Analyze Wound Healing Outcomes of Regenerative Endodontics in an Immature Premolar with Chronic Apical Abscess. J Endod. 2020 Mar 2. pii: S0099-2399(20)30041-8. doi: 10.1016/j.joen.2020.01.015.

 

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    2020-12-31 jxrzshh
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    2020-03-12 neurowu
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    2020-03-12 hunizi009
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    2020-03-10 内科新手

    谢谢梅斯提供这么好的信息,学到很多

    0

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    2020-03-10 xcdnxj

    论文写发 anni30138

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