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Int J Mol Sci：ZNF521抑制人脂肪来源的干细胞中的成骨细胞分化

2018-12-23 MedSci MedSci原创

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人脂肪来源的干细胞（hADSCs）是多能的间充质细胞，可分化成脂肪细胞，软骨细胞和骨细胞。在成骨细胞生成期间，骨祖细胞分化成成熟的成骨细胞并合成骨基质成分。锌指蛋白521（ZNF521 / Zfp521）是涉及造血，神经和间充质干细胞调节的转录辅因子，可抑制脂肪形成分化。本研究旨在确定ZNF521对hADSCs成骨细胞分化的影响，以阐明它是否可以影响其成骨分化。通过慢病毒载体转导实现了hADSCs

人脂肪来源的干细胞（hADSCs）是多能的间充质细胞，可分化成脂肪细胞，软骨细胞和骨细胞。在成骨细胞生成期间，骨祖细胞分化成成熟的成骨细胞并合成骨基质成分。锌指蛋白521（ZNF521 / Zfp521）是涉及造血，神经和间充质干细胞调节的转录辅因子，可抑制脂肪形成分化。本研究旨在确定ZNF521对hADSCs成骨细胞分化的影响，以阐明它是否可以影响其成骨分化。

通过慢病毒载体转导实现了hADSCs中ZNF521的过表达或沉默。将细胞在成骨培养基中培养长达20天。通过形态学和分子标准评估，ZNF521过表达显著降低了成骨细胞的发育，导致胶原蛋白I，碱性磷酸酶，osterix，骨桥蛋白和钙沉积物的水平降低。相反，ZNF521沉默，则刺激成骨细胞，诱导成骨早期分子标志物的显著增加，并在后期阶段，明显增强基质矿化。

综上述搜狐，该研究结果表明ZNF521抑制hADSCs中的脂肪细胞和成骨细胞成熟，其表达可能有助于维持hADSCs的不成熟特性。

原始出处：

Chiarella E, Aloisio A, et al., ZNF521 Represses Osteoblastic Differentiation in Human Adipose-Derived Stem Cells. Int J Mol Sci. 2018 Dec 18;19(12). pii: E4095. doi: 10.3390/ijms19124095.

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    2018-12-25 zhaojie88

    #成骨#

    0

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    2018-12-25 jj000004

    #骨细胞#

    0

  3. [GetPortalCommentsPageByObjectIdResponse(id=1351923, encodeId=192813519239c, content=<a href='/topic/show?id=e95954093cd' target=_blank style='color:#2F92EE;'>#成骨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=18, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54093, encryptionId=e95954093cd, topicName=成骨)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Tue Dec 25 05:16:00 CST 2018, time=2018-12-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1495113, encodeId=631514951138c, content=<a href='/topic/show?id=3c0b10182102' target=_blank style='color:#2F92EE;'>#骨细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=101821, encryptionId=3c0b10182102, topicName=骨细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=723c9147696, createdName=jj000004, createdTime=Tue Dec 25 05:16:00 CST 2018, time=2018-12-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1570246, encodeId=19ba15e0246b8, content=<a href='/topic/show?id=a66ae7809b1' target=_blank style='color:#2F92EE;'>#细胞分化#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=17, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77809, encryptionId=a66ae7809b1, topicName=细胞分化)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=653c15636865, createdName=karmond, createdTime=Tue Dec 25 05:16:00 CST 2018, time=2018-12-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=356196, encodeId=013a35619645, content=好文，值得点赞！认真学习，应用于实践！谢谢分享给广大同好！, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=eb131638261, createdName=lietome24, createdTime=Sun Dec 23 14:20:40 CST 2018, time=2018-12-23, status=1, ipAttribution=)]
    2018-12-25 karmond

    #细胞分化#

    0

  4. [GetPortalCommentsPageByObjectIdResponse(id=1351923, encodeId=192813519239c, content=<a href='/topic/show?id=e95954093cd' target=_blank style='color:#2F92EE;'>#成骨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=18, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54093, encryptionId=e95954093cd, topicName=成骨)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Tue Dec 25 05:16:00 CST 2018, time=2018-12-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1495113, encodeId=631514951138c, content=<a href='/topic/show?id=3c0b10182102' target=_blank style='color:#2F92EE;'>#骨细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=29, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=101821, encryptionId=3c0b10182102, topicName=骨细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=723c9147696, createdName=jj000004, createdTime=Tue Dec 25 05:16:00 CST 2018, time=2018-12-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1570246, encodeId=19ba15e0246b8, content=<a href='/topic/show?id=a66ae7809b1' target=_blank style='color:#2F92EE;'>#细胞分化#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=17, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77809, encryptionId=a66ae7809b1, topicName=细胞分化)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=653c15636865, createdName=karmond, createdTime=Tue Dec 25 05:16:00 CST 2018, time=2018-12-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=356196, encodeId=013a35619645, content=好文，值得点赞！认真学习，应用于实践！谢谢分享给广大同好！, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=eb131638261, createdName=lietome24, createdTime=Sun Dec 23 14:20:40 CST 2018, time=2018-12-23, status=1, ipAttribution=)]
    2018-12-23 lietome24

    好文，值得点赞！认真学习，应用于实践！谢谢分享给广大同好！

    0