J Clin Periodontol:牙周炎患者抗菌肽基因表达的初步研究

2018-05-15 MedSci MedSci原创

抗菌肽(AMPs)是先天免疫的最活跃成分之一,并有可能是牙周病发病机理的核心。本研究使用简单的牙龈涂片获取AMP编码基因以研究两组患者(慢性牙周炎患者与健康患者)之间的表达差异。

抗菌肽(AMPs)是先天免疫的最活跃成分之一,并有可能是牙周病发病机理的核心。本研究使用简单的牙龈涂片获取AMP编码基因以研究两组患者(慢性牙周炎患者与健康患者)之间的表达差异。

将23名患者分到两组,其中12名诊断为中度或重度慢性牙周炎,11名为临床健康。利用牙龈涂片提纯mRNA后使用逆转录 - 定量PCR(RT-qPCR)。

结果:使用实时RT-qPCR获得15个基因表达。与健康受试者相比,牙周炎患者中三种AMP基因,组胺3(HTN3),α-防御素4(DEFA4)和溶菌酶C(LYZ)表达水平不同。 DEFA4的相对表达水平似乎是牙周炎的保护因子。

结论:通过RT-qPCR可评估AMP编码基因的表达。缺乏DEFA4的表达可能是牙周炎状态的潜在指标。

原始出处:

Marie‐Laure Jourdain, et al. Antimicrobial peptide gene expression in periodontitis patients: A pilot study. Journal of Clinical Periodontology. 2018 May. doi:10.1111/jcpe.12879

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    2018-08-25 feather89
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    2018-06-22 jyzxjiangqin

    牙周炎患者抗菌治疗.

    0

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    2018-06-07 jyzxjiangqin

    牙周炎患者抗菌治疗.

    0

  4. 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objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cdnapi.center.medsci.cn/medsci/head/2019/12/23/2f51002e1349ccda5be11c1eea1e1e60.jpg, createdBy=a22097948, createdName=天地飞扬, createdTime=Tue May 15 23:29:42 CST 2018, time=2018-05-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=315442, encodeId=756331544204, content=^_^^_^, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/vi_32/Swp2ehfHpiciczeTntUyQOlIGMzQ705ic67sdMTVMbwTv7KbmlUvxrGvRSvQZjPkz7ib5tEDfIaORgczPtmHDpwojg/0, createdBy=c54b2163125, createdName=wqkm, createdTime=Tue May 15 22:45:45 CST 2018, time=2018-05-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=315441, encodeId=809d315441d7, content=^_^^_^^_^, beContent=null, objectType=article, channel=null, level=null, likeNumber=32, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/vi_32/Swp2ehfHpiciczeTntUyQOlIGMzQ705ic67sdMTVMbwTv7KbmlUvxrGvRSvQZjPkz7ib5tEDfIaORgczPtmHDpwojg/0, createdBy=c54b2163125, createdName=wqkm, createdTime=Tue May 15 22:45:39 CST 2018, time=2018-05-15, status=1, ipAttribution=)]
    2018-05-16 飛歌

    学习了很有用

    0

  8. 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objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cdnapi.center.medsci.cn/medsci/head/2019/12/23/2f51002e1349ccda5be11c1eea1e1e60.jpg, createdBy=a22097948, createdName=天地飞扬, createdTime=Tue May 15 23:29:42 CST 2018, time=2018-05-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=315442, encodeId=756331544204, content=^_^^_^, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/vi_32/Swp2ehfHpiciczeTntUyQOlIGMzQ705ic67sdMTVMbwTv7KbmlUvxrGvRSvQZjPkz7ib5tEDfIaORgczPtmHDpwojg/0, createdBy=c54b2163125, createdName=wqkm, createdTime=Tue May 15 22:45:45 CST 2018, time=2018-05-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=315441, encodeId=809d315441d7, content=^_^^_^^_^, beContent=null, objectType=article, channel=null, level=null, likeNumber=32, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/vi_32/Swp2ehfHpiciczeTntUyQOlIGMzQ705ic67sdMTVMbwTv7KbmlUvxrGvRSvQZjPkz7ib5tEDfIaORgczPtmHDpwojg/0, createdBy=c54b2163125, createdName=wqkm, createdTime=Tue May 15 22:45:39 CST 2018, time=2018-05-15, status=1, ipAttribution=)]
    2018-05-15 天地飞扬

    学习一下.谢谢分享!

    0

  9. 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objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cdnapi.center.medsci.cn/medsci/head/2019/12/23/2f51002e1349ccda5be11c1eea1e1e60.jpg, createdBy=a22097948, createdName=天地飞扬, createdTime=Tue May 15 23:29:42 CST 2018, time=2018-05-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=315442, encodeId=756331544204, content=^_^^_^, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/vi_32/Swp2ehfHpiciczeTntUyQOlIGMzQ705ic67sdMTVMbwTv7KbmlUvxrGvRSvQZjPkz7ib5tEDfIaORgczPtmHDpwojg/0, createdBy=c54b2163125, createdName=wqkm, createdTime=Tue May 15 22:45:45 CST 2018, time=2018-05-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=315441, encodeId=809d315441d7, content=^_^^_^^_^, beContent=null, objectType=article, channel=null, level=null, likeNumber=32, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/vi_32/Swp2ehfHpiciczeTntUyQOlIGMzQ705ic67sdMTVMbwTv7KbmlUvxrGvRSvQZjPkz7ib5tEDfIaORgczPtmHDpwojg/0, createdBy=c54b2163125, createdName=wqkm, createdTime=Tue May 15 22:45:39 CST 2018, time=2018-05-15, status=1, ipAttribution=)]
    2018-05-15 wqkm

    ^_^^_^

    0

  10. 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objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cdnapi.center.medsci.cn/medsci/head/2019/12/23/2f51002e1349ccda5be11c1eea1e1e60.jpg, createdBy=a22097948, createdName=天地飞扬, createdTime=Tue May 15 23:29:42 CST 2018, time=2018-05-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=315442, encodeId=756331544204, content=^_^^_^, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/vi_32/Swp2ehfHpiciczeTntUyQOlIGMzQ705ic67sdMTVMbwTv7KbmlUvxrGvRSvQZjPkz7ib5tEDfIaORgczPtmHDpwojg/0, createdBy=c54b2163125, createdName=wqkm, createdTime=Tue May 15 22:45:45 CST 2018, time=2018-05-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=315441, encodeId=809d315441d7, content=^_^^_^^_^, beContent=null, objectType=article, channel=null, level=null, likeNumber=32, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/vi_32/Swp2ehfHpiciczeTntUyQOlIGMzQ705ic67sdMTVMbwTv7KbmlUvxrGvRSvQZjPkz7ib5tEDfIaORgczPtmHDpwojg/0, createdBy=c54b2163125, createdName=wqkm, createdTime=Tue May 15 22:45:39 CST 2018, time=2018-05-15, status=1, ipAttribution=)]
    2018-05-15 wqkm

    ^_^^_^^_^

    0

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人β-防御素(hBD)-1是一种重要的对抗特定细菌侵入牙龈的一道防线,且葡萄糖水平与其表达相关。本研究旨在调查龈沟液(GCF)中的hBD-1水平,并比较2型糖尿病患者伴或不伴牙周炎和健康个体的表达水平。 共纳入81位受试者进行研究:伴有全身性牙周炎(T2DM + GP)的2型糖尿病(T2DM)患者21例,全身性健康广泛性牙周炎患者(GP)18例,牙周健康T2DM患者(T2DM + H)18例

J Periodontal Implant Sci:抗-P.g.IgY或可有效辅助用于牙周炎的治疗

本研究旨在评估局部使用蛋黄免疫球蛋白抗牙龈卟啉单胞菌(anti-P.g.IgY)作为治疗中度至重度慢性牙周炎中洁治和根面平整(SRP)的辅助剂的临床和微生物效应。 采用随机安慰剂对照双盲试验设计,纳入60名系统健康的中重度慢性牙周炎患者。受试者(n = 20/组)被随机分配接受SRP联合anti-P.g.IgY龈下冲洗和anti-P.g.IgY漱口水,0.2%氯己定龈下冲洗和0.2%洗必泰漱

BMJ Open Diabetes Res Care:2型糖尿病患者的牙周炎、失牙和血糖控制之间的关系

本研究旨在根据牙周和牙齿状况比较非吸烟2型糖尿病患者的血糖控制。 这项横断面研究调查了以前诊断为2型糖尿病和服用抗糖尿病药物的患者。从医学和牙科记录收集临床数据和空腹血糖(FBG)水平。根据牙齿和牙周诊断将患者分为三组:无或轻度牙周炎(NO/MILD,n = 96),中度或重度牙周炎(MOD/SEV,n = 74)和无牙颌(n = 141)。组间比较FBG的水平。Logistic回归分析也用

Int J Mol Med:双膦酸盐基质金属蛋白酶抑制剂可用于治疗牙周炎

牙周炎是一种由厌氧菌引起的炎症性疾病,包括牙龈卟啉单胞菌。脂多糖(LPS)刺激的持续炎症导致基质金属蛋白酶(MMP)表达增加,导致牙周组织破坏。本研究的目的是在暴露于LPS的人牙龈成纤维细胞组成的体外模型中研究合成的双膦酸MMP抑制剂,并比较由zoledronate(ZA)(一种商业二膦酸盐)诱导的生物学反应。分别使用MTT和乳酸脱氢酶(LDH)测定评估细胞活力和细胞毒性。使用ELISA评估前列腺

J Periodontol:IFN-λs在慢性和侵袭性牙周炎患者牙龈组织中的mRNA表达

既往研究证实慢性和侵袭性牙周炎患者牙周袋中存在疱疹病毒。最近研究发现干扰素λ(IFN-λ)具有抗病毒特性并且由疱疹病毒诱导。本研究旨在定量分析慢性和侵袭性牙周炎患者牙龈组织中IFN-λs(IFN-λ1、IFN-λ2和IFN-λ3)的mRNA表达。 研究纳入了90名参与者(男性50名,女性40名;年龄19〜50岁,平均年龄31.50±7.8岁),分为健康参与者,慢性牙周炎患者和侵袭性牙周炎患者

Clin Oral Investig:牙周治疗对伴有类风湿性关节炎的牙周炎患者的效果

本研究旨在对比非手术牙周治疗对伴有类风湿性关节炎(RA)的中重度慢性牙周炎(CP)的患者(RA-CP)和与未伴有RA的CP患者的临床和炎症指标的影响。研究共纳入了18例RA-CP患者和18例系统健康的CP患者,24小时内接受了洁治和根面平整(SRP)治疗。在基线时和SRP后3个月和6个月时,评估临床牙周参数,炎症标志物和龈下生物膜中的微生物。另外,监测RA-CP组中RA的疾病活性标记(DAS28,