J Endod:伴随根尖周炎的根充牙齿在冲洗前后牙髓感染的微生物评估

2018-01-16 lishiting MedSci原创

这篇研究的目的是通过焦磷酸测序的方法分析已根充并伴随治疗后根尖周炎的牙齿在机械化学预备以及1%次氯酸钠(NaOCl)或2%氯已定冲洗前后的根管内微生物群。

这篇研究的目的是通过焦磷酸测序的方法分析已根充并伴随治疗后根尖周炎的牙齿在机械化学预备以及1%次氯酸钠(NaOCl)或2%氯已定冲洗前后的根管内微生物群。

研究选取10颗已根充并伴随着根尖周炎的患者作为分析对象,分别在NaOCl(n = 5)或2%氯己定(n = 5)冲洗前(S1)以及冲洗后(S2)进行再治疗。提取DNA,扩增16S rRNA gene (V3-V5)可变区域以进行焦磷酸测序(GS junior 454),以此确定细菌成分。

结果显示,焦磷酸测序在S1样本中检测到43997个序列读数,在S2种检测到9196个。总的来说,共检测出属于68类(S1, 59; S2, 38)、9门共125个细菌种属。S1和S2样本中出现最多和最常见的细菌门类为硬壁菌门、梭杆菌属、拟杆菌门和放线菌。S1和S2样本中最具代表性、最多以及最常见的菌属为链球菌属和梭杆菌属。S1和S2样本中最流行的种属为具核梭杆菌vincentii、链球菌oralis/mitis、中链球菌和格氏链球菌。每个根管的平均种属数量S1为20 种(range, 4-37),S2为9种 (range, 4-15)。

结论:在S1和S2样本间存在较高的个体多样性,并且在2组冲洗液中未发现显著性差异。在已根充并有炎症牙齿的再治疗过程中,具核梭杆菌vincentii和一些链球菌属是在预备前后最为普遍出现的菌属。

原始出处

Zandi H, Kristoffersen AK, et al. Microbial Analysis of Endodontic Infections in Root-filled Teeth with Apical Periodontitis before and after Irrigation Using Pyrosequencing. J Endod. 2018 Jan 3. pii: S0099-2399(17)31264-5. doi: 10.1016/j.joen.2017.11.019.

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    2018-03-20 jxrzshh
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    2018-01-18 zhouqu_8
  7. [GetPortalCommentsPageByObjectIdResponse(id=1824873, encodeId=35dd18248e332, content=<a href='/topic/show?id=702a5065860' target=_blank style='color:#2F92EE;'>#微生物#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=33, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=50658, encryptionId=702a5065860, topicName=微生物)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=0b7c20, createdName=wjcjjian, createdTime=Thu Nov 29 08:38:00 CST 2018, time=2018-11-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1669633, encodeId=098c166963350, content=<a href='/topic/show?id=c7ca6e660ab' target=_blank style='color:#2F92EE;'>#牙髓#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=34, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67660, encryptionId=c7ca6e660ab, topicName=牙髓)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=5eeb26562774, createdName=jxrzshh, createdTime=Tue Mar 20 10:38:00 CST 2018, time=2018-03-20, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1739929, encodeId=33381e3992977, content=<a href='/topic/show?id=f49c310e134' target=_blank style='color:#2F92EE;'>#冲洗#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=49, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=31071, encryptionId=f49c310e134, topicName=冲洗)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=4ec334602181, createdName=ms3892185887221420, createdTime=Mon Aug 06 15:38:00 CST 2018, time=2018-08-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1927338, encodeId=ae06192e3382a, content=<a href='/topic/show?id=5a4d6e672eb' target=_blank style='color:#2F92EE;'>#牙髓感染#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=34, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67672, encryptionId=5a4d6e672eb, topicName=牙髓感染)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=052a130, createdName=yyj062, createdTime=Sun Oct 21 16:38:00 CST 2018, time=2018-10-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1380204, encodeId=f88913802043a, content=<a href='/topic/show?id=48786219204' target=_blank style='color:#2F92EE;'>#根尖周炎#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=39, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=62192, encryptionId=48786219204, topicName=根尖周炎)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=fcae472, createdName=zhangbaojun, createdTime=Thu Jan 18 11:38:00 CST 2018, time=2018-01-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1430116, encodeId=4484143011668, content=<a href='/topic/show?id=b58f6e691fc' target=_blank style='color:#2F92EE;'>#牙齿#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67691, encryptionId=b58f6e691fc, topicName=牙齿)], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/aLGWoFXAyMbIu3qymFOyheQLjPSX3OUs5GmkyBlcCOwTPIeq3why9NGibxxUqYo6hcx8qZLHZFgNPnBK1yzWeOFpyg2OnWOt0/0, createdBy=fa4716, createdName=zhouqu_8, createdTime=Thu Jan 18 11:38:00 CST 2018, time=2018-01-18, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=279510, encodeId=c7c02e9510eb, content=是很好的学习材料.不错.以后会多学习., beContent=null, objectType=article, channel=null, level=null, likeNumber=53, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/jW482SpianMayicTRbRZ5RzUn81b5CF5ibVPib8jWI92iciaxmqGHlwruOnK4SVZykd4iahqo1hicklIibyZoxm55X7EM8BW9eX1h1hQ2/0, createdBy=8aa81937526, createdName=李东泽, createdTime=Wed Jan 17 22:02:00 CST 2018, time=2018-01-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=279185, encodeId=d1962e91859f, content=是很好的学习材料.不错.以后会多学习., beContent=null, objectType=article, channel=null, level=null, likeNumber=52, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/jW482SpianMayicTRbRZ5RzUn81b5CF5ibVPib8jWI92iciaxmqGHlwruOnK4SVZykd4iahqo1hicklIibyZoxm55X7EM8BW9eX1h1hQ2/0, createdBy=8aa81937526, createdName=李东泽, createdTime=Tue Jan 16 20:58:07 CST 2018, time=2018-01-16, status=1, ipAttribution=)]
    2018-01-17 李东泽

    是很好的学习材料.不错.以后会多学习.

    0

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    2018-01-16 李东泽

    是很好的学习材料.不错.以后会多学习.

    0

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