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J Appl Oral Sci：视黄酸增加骨形态发生蛋白2型对人脂肪来源干细胞成骨分化的影响

2019-03-14 不详 网络

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骨形态发生蛋白2型（BMP-2）和维甲酸（RA）是刺激内源性骨修复机制的骨诱导因子，可用于口腔和颌面部骨质缺损的处理。考虑到RA对成骨的不同结果及其替代/效力BMP-2效应的可能用途，本研究评估了BMP-2，RA和BMP-2 + RA治疗对人脂肪来源干细胞（ASCs）的体外成骨分化和涉及的信号通路的影响。每隔一天用单独的基础成骨培养基（OM）或补充BMP-2，RA或BMP-2 + RA治疗ASC。

骨形态发生蛋白2型（BMP-2）和维甲酸（RA）是刺激内源性骨修复机制的骨诱导因子，可用于口腔和颌面部骨质缺损的处理。考虑到RA对成骨的不同结果及其替代/效力BMP-2效应的可能用途，本研究评估了BMP-2，RA和BMP-2 + RA治疗对人脂肪来源干细胞（ASCs）的体外成骨分化和涉及的信号通路的影响。

每隔一天用单独的基础成骨培养基（OM）或补充BMP-2，RA或BMP-2 + RA治疗ASC。使用r-硝基苯酚法测定碱性磷酸酶（ALP）活性。使用von Kossa染色和钙定量评估细胞外基质矿化。使用qPCR测定骨粘连蛋白和骨钙蛋白mRNA的表达。使用蛋白质印迹分析Smad1，Smad4，磷酸化的Smad1/5/8，BMP-4和BMP-7蛋白表达。使用IPA®软件评估信号传导通路。

结果显示，与BMP-2和BMP-2 + RA相比，RA在第7,14,21和28天的ALP活性较高。BMP-2 + RA在第7天最佳刺激磷酸化Smad1/5/8蛋白表达，在第7,14,21和28天最佳刺激Smad4表达。在第7天，BMP-2 + RA最好地刺激骨钙蛋白和骨粘连蛋白mRNA表达。在第12天和第32天，BMP-2 + RA最大程度地改善了基质矿化。此外，BMP-2 + RA在第7天和第14天促进了最高的BMP信号通路活化，并且表现出比仅OM更多的骨形成细胞分化活化。

总之，该研究结果表明，RA增加了BMP-2对人ASCs成骨分化的影响。

原始出处：

Cruz ACC, Cardozo FTGS, et al., Retinoic acid increases the effect of bone morphogenetic protein type 2 on osteogenic differentiation of human adipose-derived stem cells. J Appl Oral Sci. 2019 Feb 21;27:e20180317. doi: 10.1590/1678-7757-2018-0317.

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    2019-10-05 HinsMax

    #骨分化#

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    2019-11-10 小几洁

    #Oral#

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    2019-05-18 ms1467340776200723

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    2019-03-16 ms5880526374914242

    #成骨分化#

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    2019-03-16 zhaojie88

    #成骨#

    0