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Assessment of memory formation by metabolically engineered antigen-specific CD8 T cells

期刊: TUMOR IMMUNOLOGY AND IMMUNOTHERAPY - CELLULAR METHODS, PT A, 2020; 631 ()

The formation of memory CD8(+) T cells is crucial for host protection upon reencounter of the same pathogen. Moreover, long-lasting anti-tumoral effec......

Quantitative determination of phagocytosis by bone marrow-derived dendritic cells via imaging flow cytometry

期刊: TUMOR IMMUNOLOGY AND IMMUNOTHERAPY - CELLULAR METHODS, PT B, 2020; 632 ()

Immunogenic cell death (ICD), induced by certain anticancer chemotherapeutics, leads to the emission of danger associated molecular patterns (DAMP) by......

Side-by-side comparison of flow cytometry and immunohistochemistry for detection of calreticulin exposure in the course of immunogenic cell death

期刊: TUMOR IMMUNOLOGY AND IMMUNOTHERAPY - CELLULAR METHODS, PT B, 2020; 632 ()

Immunogenic cell death (ICD), a functionally peculiar type of apoptosis, represents a unique way to deliver danger-associated molecular patterns (DAMP......

Quantitation of calreticulin exposure associated with immunogenic cell death

期刊: TUMOR IMMUNOLOGY AND IMMUNOTHERAPY - CELLULAR METHODS, PT B, 2020; 632 ()

Tumor cells treated by immunogenic cell death (ICD) inducers emit danger associated molecular patterns (DAMP), including but not limited to calreticul......

The NMR tube bioreactor

期刊: CHEMICAL AND SYNTHETIC BIOLOGY APPROACHES TO UNDERSTAND CELLULAR FUNCTIONS - PT C, 2020; 633 ()

Enzymes are pliable systems and core cellular components allowing the performance of several processes. They can also be utilized as "green" synthetic......

Optically guided mass spectrometry to screen microbial colonies for directed enzyme evolution

期刊: ENZYME ENGINEERING AND EVOLUTION: SPECIFIC ENZYME APPLICATIONS, 2020; 644 ()

Directed evolution is a well-established and powerful tool for enzyme engineering, which consists of iterative rounds of creating and screening a libr......

Focused rational iterative site-specific mutagenesis (FRISM)

期刊: ENZYME ENGINEERING AND EVOLUTION: GENERAL METHODS, 2020; 643 ()

Directed evolution has emerged as the most productive enzyme engineering method, with stereoselectivity playing a crucial role when evolving mutants f......

Remodeling enzyme active sites by stepwise loop insertion

期刊: ENZYME ENGINEERING AND EVOLUTION: GENERAL METHODS, 2020; 643 ()

The remolding active site loops via residue insertion/deletion as well as substitution is thought to play a key role in enzyme divergent evolution. Ho......

Dynamic nuclear polarization enhanced neutron crystallography: Amplifying hydrogen in biological crystals

期刊: NEUTRON CRYSTALLOGRAPHY IN STRUCTURAL BIOLOGY, 2020; 634 ()

Dynamic nuclear polarization (DNP) can provide a powerful means to amplify neutron diffraction from biological crystals by 10-100-fold, while simultan......

Identification of lamins as the molecular targets of LBL1 using a clickable photoaffinity probe

期刊: CHEMICAL AND SYNTHETIC BIOLOGY APPROACHES TO UNDERSTAND CELLULAR FUNCTIONS - PT C, 2020; 633 ()

Phenotypic screening is a powerful approach to discover small molecules targeting pathways or disease biology with complex genetic causes. Following t......

A suite of bioassays to evaluate CREB inhibitors

期刊: CHEMICAL AND SYNTHETIC BIOLOGY APPROACHES TO UNDERSTAND CELLULAR FUNCTIONS - PT C, 2020; 633 ()

The cyclic-AMP response element binding protein (CREB) is an important nuclear transcription factor and has been shown to be overexpressed and/or over......

Chemical synthesis of di-ubiquitin modified histones for further biochemical studies

期刊: CHEMICAL TOOLS FOR IMAGING, MANIPULATING, AND TRACKING BIOLOGICAL SYSTEMS: DIVERSE METHODS FOR OPTICAL IMAGING AND CONJUGATION, 2020; 639 ()

Histone ubiquitination plays critical roles in almost all the DNA-related processes, such as DNA replication, transcription and repair. The various ou......

Ortho-Phthalaldehyde (OPA)-based chemoselective protein bioconjugation and peptide cyclization

期刊: CHEMICAL TOOLS FOR IMAGING, MANIPULATING, AND TRACKING BIOLOGICAL SYSTEMS: DIVERSE METHODS FOR OPTICAL IMAGING AND CONJUGATION, 2020; 639 ()

Ortho-Phthalaldehyde (OPA)-amine reaction and OPA-amine-thiol reaction have been developed to effectively modify native peptides and proteins under th......

Washington Red (WR) dyes and their imaging applications

期刊: CHEMICAL TOOLS FOR IMAGING, MANIPULATING, AND TRACKING BIOLOGICAL SYSTEMS: DIVERSE METHODS BASED ON OPTICAL IMAGING AND FLUORESCENCE, 2020; 640 ()

Near-infrared (NIR) fluorescent dyes with optimum photophysical properties are highly useful for bioimaging in living systems, but such dyes are still......

Three-dimensional cell models for extracellular vesicles production, isolation, and characterization

期刊: EXTRACELLULAR VESICLES, 2020; 645 ()

Extracellular vesicles (EVs) play a pivotal role in cancer progression. However, the majority of functional studies performed so far relies on data ac......

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