Remodeling enzyme active sites by stepwise loop insertion

Hoque, MA; Zhang, Y; Li, Z; Cui, L; Feng, Y

Feng, Y (corresponding author), Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, State Key Lab Microbial Metab, Shanghai, Peoples R China.

ENZYME ENGINEERING AND EVOLUTION: GENERAL METHODS, 2020; 643 (): 111

Abstract

The remolding active site loops via residue insertion/deletion as well as substitution is thought to play a key role in enzyme divergent evolution. Ho......

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