Light-Induced Stomatal Opening in Wild-Type and Mutant rop2 Arabidopsis.
(A) Light-induced stomatal opening in CA-rop2, wild-type (ecotype Columbia [Col-0]), and DN-rop2 Arabidopsis.
(B) Gene structure of ROP2 and site of the T-DNA insertion in the rop2 knockout line in rop2-1. Boxes represent exons. Arrows indicate the regions of ROP2 from which the ROP2-specific primers were designed for RT-PCR. pROK2, T-DNA present in the SALK Arabidopsis mutants.
(C) RT-PCR amplification of ROP2 mRNA. ROP2 transcript was amplified from wild-type and three independent complemented lines (ROP2-1, ROP2-2, and ROP2-3) but not from rop2-1. Tubulin was amplified as a positive control.
(D) Light-induced stomatal opening in wild-type (Col-0), rop2-1, and three independent lines of ROP2-complemented rop2-1 Arabidopsis. White light with an intensity of 250 μmol·m−2·s−1 was used to irradiate the plants. Results (means ± se; n > 250) from four independent experiments are shown. In each data set, the extent of increase in stomatal aperture and the time to reach half-maximum stomatal aperture (t1/2) were obtained by fitting the data from illuminated samples to a first-order exponential function.
