笔记详情
标题
Metformin induced AMPK and SIRT1
内容
Metformin induced AMPK and SIRT1
AMPK and SIRT are 2 enzymes involved in regulation of energy metabolism and reported to mediate the positive effects of CR [16, 25]. The CRD group showed the strongest activation of phosphorylated acetyl-CoA carboxylase (pACC), a surrogate marker for AMPK activation, while HED groups demonstrated almost no phosphorylation of ACC as reported before [13] (Figure 6A, 6B). Metformin increased the pACC expression significantly in RD and HED groups in both peritoneal and adipose tumor tissue. A similar pattern was also observed for SIRT1 expression in the tumor tissues (Figure 6D, 6E). Metformin treatment also activated AMPK and SIRT1 in the liver, which was also associated with the amelioration of the hepatic steatosis observed in the HED group (Figure 6C, 6F). The quantification of the staining intensity is represented as bar graphs (0–1: no or weak stain; 2: moderate stain and 3: strong stain), respective of each panel. Taken together, these results suggest that metformin activated AMPK and increased the expression of SIRT1 significantly, parallel to CRD both in the tumor and in the host tissue. 点击翻译
AMPK and SIRT are 2 enzymes involved in regulation of energy metabolism and reported to mediate the positive effects of CR [16, 25]. The CRD group showed the strongest activation of phosphorylated acetyl-CoA carboxylase (pACC), a surrogate marker for AMPK activation, while HED groups demonstrated almost no phosphorylation of ACC as reported before [13] (Figure 6A, 6B). Metformin increased the pACC expression significantly in RD and HED groups in both peritoneal and adipose tumor tissue. A similar pattern was also observed for SIRT1 expression in the tumor tissues (Figure 6D, 6E). Metformin treatment also activated AMPK and SIRT1 in the liver, which was also associated with the amelioration of the hepatic steatosis observed in the HED group (Figure 6C, 6F). The quantification of the staining intensity is represented as bar graphs (0–1: no or weak stain; 2: moderate stain and 3: strong stain), respective of each panel. Taken together, these results suggest that metformin activated AMPK and increased the expression of SIRT1 significantly, parallel to CRD both in the tumor and in the host tissue. 点击翻译
来源
Zaid Al-Wahab. Oncotarget. 2015 Mar 12
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