笔记详情
标题
Metformin decreases the tumor burden and ascites volume
内容
Metformin decreases the tumor burden and ascites volume
To investigate if metformin can reduce tumor progression similar to CR, a set of HED and regular diet (RD) fed mice were given metformin daily in drinking water 7 days after tumor implantation. As previously reported [13], mice on HED had highest weight gain while the CR diet (CRD) mice maintained their weight (Figure 1A). Metformin intake did not significantly affect the weight gain of HED or RD mice (Figure 1A), which was also reflected in the end weights at the time of the sacrifice (Figure 1B). Metformin treatment significantly reduced the ascites accumulation in both RD and HED groups, but the highest reduction was observed in the CRD group (Figure 1C). Metformin treatment of the HED group was most effective in reducing the tumor progression with significant reduction of tumor burden in the peritoneum, diaphragm, bowel, liver, kidney and spleen (Figure 2A–2F). In the RD group, metformin reduced the tumor burden at the bowel, liver, kidney and spleen. CRD group had the lowest tumor burden at all organ sites (Figure 2A–2F). The hemotoxylin and eosin evaluation of the tumor sections also showed reduced tumor nodules at the diaphragm, peritoneum and adipose tissue (Figure 3A–3C) with metformin treatment in both HED and RD groups, akin to CRD group. This was also reflected in the decreased number of positive Ki-67 stained cells observed in tumors from CRD and metformin treated groups, quantified as Ki-67 index (Figure 3D). Overall, our data showed that metformin decreased the ascites and tumor burden in both the RD and HED groups significantly, similar to CRD. However, the tumor reduction by metformin in HED group was more pronounced than the tumor reduction observed in the RD treatment group. 点击翻译
To investigate if metformin can reduce tumor progression similar to CR, a set of HED and regular diet (RD) fed mice were given metformin daily in drinking water 7 days after tumor implantation. As previously reported [13], mice on HED had highest weight gain while the CR diet (CRD) mice maintained their weight (Figure 1A). Metformin intake did not significantly affect the weight gain of HED or RD mice (Figure 1A), which was also reflected in the end weights at the time of the sacrifice (Figure 1B). Metformin treatment significantly reduced the ascites accumulation in both RD and HED groups, but the highest reduction was observed in the CRD group (Figure 1C). Metformin treatment of the HED group was most effective in reducing the tumor progression with significant reduction of tumor burden in the peritoneum, diaphragm, bowel, liver, kidney and spleen (Figure 2A–2F). In the RD group, metformin reduced the tumor burden at the bowel, liver, kidney and spleen. CRD group had the lowest tumor burden at all organ sites (Figure 2A–2F). The hemotoxylin and eosin evaluation of the tumor sections also showed reduced tumor nodules at the diaphragm, peritoneum and adipose tissue (Figure 3A–3C) with metformin treatment in both HED and RD groups, akin to CRD group. This was also reflected in the decreased number of positive Ki-67 stained cells observed in tumors from CRD and metformin treated groups, quantified as Ki-67 index (Figure 3D). Overall, our data showed that metformin decreased the ascites and tumor burden in both the RD and HED groups significantly, similar to CRD. However, the tumor reduction by metformin in HED group was more pronounced than the tumor reduction observed in the RD treatment group. 点击翻译
来源
Zaid Al-Wahab. Oncotarget. 2015 Mar 12
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