HGF does not block p65 NF-κB nuclear translocation but abolishes its binding to the cognate cis-acting element in RANTES promoter. A and B: HGF did not block the p65 NF-κB nuclear translocation induced by TNF-α. HKC-8 cells were treated with TNF-α or/and HGF as indicated. A: Representative micrographs showed the staining for p65 NF-κB after various treatments. B: The percentages of cell population with p65 nuclear translocation after various treatments are given. *P < 0.05 versus control. C: ChIP assay demonstrated that HGF signaling abolished the binding of p65 to its cis-acting element in the RANTES promoter. Marker, DNA size markers; IgG, precipitated with control IgG.