笔记详情
标题
Heterogeneity of EGFR mutation detected by DHPLC and ARMS
内容
Heterogeneity of EGFR mutation detected by DHPLC and ARMS
Tumor microdissection yielded 2,699 foci, including 1,238 foci from wild-type EGFR (group-W) and 1,431 group-M cases. The overall mutant frequency in group-M was 80.6% by DHPLC (1,154/1,431) and 87.1% (1,247/1,431) by ARMS. Mutant frequencies varied widely throughout individual tumors, ranging between 5%–100% by DHPLC and 1%–100% by ARMS. Group-M samples were subdivided by mutation content as follows: 1) pure EGFR mutation (100%) or no mutational heterogeneity was detected in 21 cases by DHPLC (12 group-M/E19, 9 group-M/E21) and in 31 cases by ARMS (20 group-M/E19, 11 group-M/E21); 2) moderate mutant content (≥50%) or moderate-level heterogeneity was detected in 16 cases by DHPLC (10 group-M/E19, 6 group-M/E21) and in 9 cases by ARMS (2 group-M/E19, 7 group-M/E21); and 3) low mutant content or high-level heterogeneity (<50%) were detected in 8 cases by DHPLC (3 group-M/E19 and 5 group-M/E21) and in 5 cases by ARMS (3 group-M/E19 and 2 group-M/E21).
Among the 40 group-W cases, 4 cases displayed low mutant frequencies ranging from 5.0% to 8.0% when microdissected tumor foci were analyzed by DHPLC. Three of these cases carried an EGFR exon 19 mutation, and one case carried an EGFR exon 21 mutation. ARMS confirmed these 4 cases and identified 6 additional cases showing very low mutant frequencies ranging from 1.0% to 5.0%. By combining the group-M cases carrying both wild- and mutant-type EGFR cells and the group-W cases containing mutant cells at low frequency, 32.9% (28/85) and 28.2% (24/85) of samples were identified to carry intratumoral EGFR mutational heterogeneity by DHPLC and ARMS, respectively. Difference of intratumoral EGFR mutational heterogeneity identified by two methods was not statistical significance (P = 0.031, McNemar's test) (Figure 1). 点击翻译
Tumor microdissection yielded 2,699 foci, including 1,238 foci from wild-type EGFR (group-W) and 1,431 group-M cases. The overall mutant frequency in group-M was 80.6% by DHPLC (1,154/1,431) and 87.1% (1,247/1,431) by ARMS. Mutant frequencies varied widely throughout individual tumors, ranging between 5%–100% by DHPLC and 1%–100% by ARMS. Group-M samples were subdivided by mutation content as follows: 1) pure EGFR mutation (100%) or no mutational heterogeneity was detected in 21 cases by DHPLC (12 group-M/E19, 9 group-M/E21) and in 31 cases by ARMS (20 group-M/E19, 11 group-M/E21); 2) moderate mutant content (≥50%) or moderate-level heterogeneity was detected in 16 cases by DHPLC (10 group-M/E19, 6 group-M/E21) and in 9 cases by ARMS (2 group-M/E19, 7 group-M/E21); and 3) low mutant content or high-level heterogeneity (<50%) were detected in 8 cases by DHPLC (3 group-M/E19 and 5 group-M/E21) and in 5 cases by ARMS (3 group-M/E19 and 2 group-M/E21).
Among the 40 group-W cases, 4 cases displayed low mutant frequencies ranging from 5.0% to 8.0% when microdissected tumor foci were analyzed by DHPLC. Three of these cases carried an EGFR exon 19 mutation, and one case carried an EGFR exon 21 mutation. ARMS confirmed these 4 cases and identified 6 additional cases showing very low mutant frequencies ranging from 1.0% to 5.0%. By combining the group-M cases carrying both wild- and mutant-type EGFR cells and the group-W cases containing mutant cells at low frequency, 32.9% (28/85) and 28.2% (24/85) of samples were identified to carry intratumoral EGFR mutational heterogeneity by DHPLC and ARMS, respectively. Difference of intratumoral EGFR mutational heterogeneity identified by two methods was not statistical significance (P = 0.031, McNemar's test) (Figure 1). 点击翻译
来源
PLoS One. 2013; 8(2): e54170.
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