ABA2 overexpression lines delay seed germination. A, Germination of wild type and overexpression lines without cold treatment. Seeds were grown on 1% Suc agar plates for the times shown. Radicle emergence over 1 mm is referred to as germination. B, Germination of wild type and overexpression lines with cold pretreatment for 3 d at 4°C. The seed growth condition was the same as in A. C, Germination of wild type, aba2, and overexpression lines on 2% Glc agar plates. D, Germination of wild type, aba2, and overexpression lines on 6% Glc agar plates. E, Phenotypes of wild type, aba2, and overexpression lines grown on 6% Glc agar plates with or without 1 μm fluridone for 14 d. Germination rates from A to D were counted as germinated seeds/total germinated seeds at day 10. The results shown were the means ± sd of three independent experiments using different seed batches, with consistent results, each with 200 to 250 seeds. The seeds in B, C, D, and E were subjected to cold pretreatment at 4°C for 3 d before planting; in A, without cold treatment. WT, Wild type. [See online article for color version of this figure.]