Generation of genetically modified mice using SpCas9-NG engineered nuclease

Fujii, W; Ito, H; Kanke, T; Ikeda, A; Sugiura, K; Naito, K

Fujii, W (reprint author), Univ Tokyo, Grad Sch Agr & Life Sci, Dept Anim Resource Sci, Bunkyo Ku, 1-1-1 Yayoi, Tokyo 1138657, Japan.

SCIENTIFIC REPORTS, 2019; 9 ():

Abstract

Although genetically modified mice can be generated with high efficiency by using CRISPR/Cas9-mediated genome editing in mouse zygotes, only the loci ......

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