Engineered dual selection for directed evolution of SpCas9 PAM specificity

Goldberg, GW; Spencer, JM; Giganti, DO; Camellato, BR; Agmon, N; Ichikawa, DM; Boeke, JD; Noyes, MB

Goldberg, GW; Noyes, MB (corresponding author), NYU, Inst Syst Genet, Langone Hlth, New York, NY 10016 USA.

NATURE COMMUNICATIONS, 2021; 12 (1):

Abstract

The widely used Streptococcus pyogenes Cas9 (SpCas9) nuclease derives its DNA targeting specificity from protein-DNA contacts with protospacer adjacen......

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