Lowering DNA binding affinity of SssI DNA methyltransferase does not enhance the specificity of targeted DNA methylation in E. coli

Slaska-Kiss, K; Zsibrita, N; Koncz, M; Albert, P; Csabradi, A; Szentes, S; Kiss, A

Kiss, A (corresponding author), Eotvos Lorand Res Network ELKH, Biol Res Ctr, Inst Biochem, Lab DNA Prot Interact, Temesvari Krt 62, H-6726 Szeged, Hungary.

SCIENTIFIC REPORTS, 2021; 11 (1):

Abstract

Targeted DNA methylation is a technique that aims to methylate cytosines in selected genomic loci. In the most widely used approach a CG-specific DNA ......

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