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J Endod：二甲双胍介导AMPK通路增强牙髓细胞向成牙本质细胞的分化

2018-04-21 lishiting MedSci原创

二甲双胍牙髓细胞分化矿化
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二甲双胍是治疗II型糖尿病的一线药物，它可以调节间充质干细胞的分化。然而，它对人牙髓细胞(DPCs)的作用还未可知。这篇研究的目的是为了调查二甲双胍对DPCs增殖和分化的作用。

二甲双胍是治疗II型糖尿病的一线药物，它可以调节间充质干细胞的分化。然而，它对人牙髓细胞(DPCs)的作用还未可知。这篇研究的目的是为了调查二甲双胍对DPCs增殖和分化的作用。研究使用活/死活力测定试剂盒检测二甲双胍对DPCs细胞活力的影响。使用细胞计数试剂盒(CCK-8； Dojindo， Tokyo， Japan)检测细胞增殖情况。在施加二甲双胍和腺苷5'-单磷酸酯活化的蛋白激酶（AMPK）信号抑制剂Compound C (EMD Chemicals， San Diego， CA)的情况下，通过western blot检测AMPK的磷酸化及非磷酸化水平。通过碱性磷酸酶活性和von Kossa染色检测Compound C对二甲双胍诱导的DPCs的成牙本质细胞分化的作用，并通过反转录聚合酶链式反应检测成牙本质分化标志物的表达变化。结果显示，流式细胞仪检测结果表明，DPCs展现出间充质干细胞的特性。不同剂量的二甲双胍均展现出与DPCs良好的相容性，细胞活力>90%。高达50μmol/ L的二甲双胍浓度不会影响细胞增殖。Western blot结果显示，DPCs表达功能性的有机阳离子

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    2018-12-25 jxrzshh

    #牙髓#

    0

  2. [GetPortalCommentsPageByObjectIdResponse(id=1669661, encodeId=9e07166966179, content=<a href='/topic/show?id=c7ca6e660ab' target=_blank style='color:#2F92EE;'>#牙髓#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=38, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67660, encryptionId=c7ca6e660ab, topicName=牙髓)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=5eeb26562774, createdName=jxrzshh, createdTime=Tue Dec 25 06:06:00 CST 2018, time=2018-12-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1724349, encodeId=81441e243494d, content=<a href='/topic/show?id=e1f56e68935' target=_blank style='color:#2F92EE;'>#牙髓细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=37, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67689, encryptionId=e1f56e68935, topicName=牙髓细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=7f2d33606535, createdName=mnda, createdTime=Sun Jul 08 15:06:00 CST 2018, time=2018-07-08, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1441895, encodeId=34be144189524, content=<a href='/topic/show?id=67526e55924' target=_blank style='color:#2F92EE;'>#牙本质#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=36, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67559, encryptionId=67526e55924, topicName=牙本质)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=37364903098, createdName=ms8538335253054589, createdTime=Mon Apr 23 08:06:00 CST 2018, time=2018-04-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1468183, encodeId=4da0146818334, content=<a href='/topic/show?id=71c810212946' target=_blank style='color:#2F92EE;'>#髓细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=36, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=102129, encryptionId=71c810212946, topicName=髓细胞)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=69e46898758, createdName=chengjn, createdTime=Mon Apr 23 08:06:00 CST 2018, time=2018-04-23, status=1, ipAttribution=)]
    2018-07-08 mnda

    #牙髓细胞#

    0

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    2018-04-23 ms8538335253054589

    #牙本质#

    0

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    2018-04-23 chengjn

    #髓细胞#

    0

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