STM:美学者揭示寨卡病毒快速检测工具

2017-05-22 生物帮 生物帮

2017年5月5日,国际顶尖学术期刊《Science》旗下《 Science Translational Medicine》杂志上在线发表了美国科罗拉多州立大学Joel Rovnak研究员的一篇研究论文,论文报道了一种快速、高灵敏且便宜的新型检测工具,不仅能直接检测到蚊子体内和人体体液中的寨卡病毒,还能区分寨卡病毒的非洲株和亚洲株,可更有效地追踪寨卡病毒的传播。

2017年5月5日,国际顶尖学术期刊《Science》旗下《 Science Translational Medicine》杂志上在线发表了美国科罗拉多州立大学Joel Rovnak研究员的一篇研究论文,论文报道了一种快速、高灵敏且便宜的新型检测工具,不仅能直接检测到蚊子体内和人体体液中的寨卡病毒,还能区分寨卡病毒的非洲株和亚洲株,可更有效地追踪寨卡病毒的传播。

寨卡病毒是一种虫媒传播病毒,与登革热、黄热病和西尼罗河病毒同属。寨卡病毒属于单链RNA病毒,分为亚洲系和非洲系两大谱系。感染后常见症状包括发烧、疹子、关节疼痛、肌肉疼痛、头痛和结膜炎等。孕妇感染可导致婴儿小头症等严重出生缺陷。

研究人员设计的新工具,基于一种叫做“环介导等温扩增”技术(LAMP),能直接从蚊子体内以及人的血液、唾液和精液中检测到寨卡病毒,其敏感性堪比目前的标准PCR(聚合酶链反应)检测技术。但PCR仪器的价格从1.5万美元至2.5万美元不等,仅在实验室内使用;而新工具初步估计成本为250美元左右,能在野外使用。

研究人员利用人为掺入病毒的健康人样本及寨卡病毒感染者身上采集的临床样本,验证了LAMP的有效性。LAMP还足够灵敏,能从50只蚊虫中找出唯一受到感染的蚊子。此外,LAMP对样本处理的要求很低,而检测时间却缩短很多,这有利于对寨卡病毒的监控。更重要的是,它不会把登革病毒和基孔肯雅病毒等与寨卡病毒相似的病原体误检测成寨卡病毒。

不过,研究人员表示,尽管这种新工具对监控寨卡疫情很有价值,但要通过审批进而大范围使用,估计还要很长时间。

原始出处:

Nunya Chotiwan, Connie D. Brewster, et al. Rapid and specific detection of Asian- and African-lineage Zika viruses. Science Translational Medicine  03 May 2017: Vol. 9, Issue 388, eaag0538 DOI: 10.1126/scitranslmed.aag0538

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    2017-08-28 docwu2019
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    2017-06-28 gdsun
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    2017-05-23 吴教授

    学习了

    0

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    2017-05-22 王秀

    学习了不错

    0

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    2017-05-22 daiyaozu

    学习了,不错

    0

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