J Periodontal Res:吸烟习惯影响种植体周微生物的组成

2018-10-17 MedSci MedSci原创

吸烟是种植体周围疾病的公认风险因素,并导致粘膜炎和种植体周围炎的微生物变化。然而,目前尚不清楚吸烟对健康的种植体周围组织的影响。本研究旨在评估健康种植体的吸烟者其种植体周的微生物组。 在12名非吸烟者(NSMK)和12名吸烟者(SMK)非牙周炎患者(无出血,探查深度<4 mm)的单个临床健康,螺钉固位,牙齿包围的植体周围收集种植体周围生物膜。分离细菌DNA,并使用焦磷酸测序对16S核糖

吸烟是种植体周围疾病的公认风险因素,并导致粘膜炎和种植体周围炎的微生物变化。然而,目前尚不清楚吸烟对健康的种植体周围组织的影响。本研究旨在评估健康种植体的吸烟者其种植体周的微生物组。

在12名非吸烟者(NSMK)和12名吸烟者(SMK)非牙周炎患者(无出血,探查深度<4 mm)的单个临床健康,螺钉固位,牙齿包围的植体周围收集种植体周围生物膜。分离细菌DNA,并使用焦磷酸测序对16S核糖体RNA基因文库进行测序,靶向V3-V4区域。使用Quantitative Insights into Microbial Ecology,Greengenes和Human Oral Microbiome Database数据库处理数据集。

结果显示,与NSMK微生物组相比,SMK种植体周围微生物组存在明显差异,即使是健康的种植体周围,也具有大量菌种。 SMK核心-微生物组显示出丰富的梭杆菌属,Tannerella和Mogibacterium,而NSMK核心显示出大量的放线菌,Capnocytophaga和Streptococcus属,通常与牙周健康有关。微生物组相互关系在SMK中显示出更好的通用性,然后在NSMK中,表明不同的微生物组内聚。

综上所述,该研究结果表明,吸烟对种植体外微生物组产生负面影响,导致与疾病相关的状态,即使在临床健康的个体中也是如此。

原始出处:

Pimentel SP, Fontes M, et al., Smoking habit modulates peri-implant microbiome: A case-control study. J Periodontal Res. 2018 Sep 26. doi: 10.1111/jre.12597. 

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    2019-03-07 feather89
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    2018-12-08 huangdf
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    2018-10-17 div

    梭杆菌聚集

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