Nat Commun:石墨烯大用途——高效高通量DNA测序

2015-04-29 佚名 生物谷

近日,刊登在国际杂志Nature Communications上的一篇研究论文中,来自墨尔本大学的研究人员通过研究开发了一种新技术,其可以利用特殊形式的碳来进行实时高通量的DNA测序,该技术或为医学研究和检测带来革命性的进步。 研究者Jiri Cervenka博士表示,石墨烯是一种单原子层的六角形排列碳,其形状类似于鸡爪样,其可以帮助检测组成DNA的四种核酸碱基(A,T,C,G),四种碱基的

近日,刊登在国际杂志Nature Communications上的一篇研究论文中,来自墨尔本大学的研究人员通过研究开发了一种新技术,其可以利用特殊形式的碳来进行实时高通量的DNA测序,该技术或为医学研究和检测带来革命性的进步。

研究者Jiri Cervenka博士表示,石墨烯是一种单原子层的六角形排列碳,其形状类似于鸡爪样,其可以帮助检测组成DNA的四种核酸碱基(A,T,C,G),四种碱基的特殊组合就组成了DNA序列,当前DNA测序是一种最基本的工具,可以帮助科学家们进行医学诊断、法医检测以及医学生物学研究等。

相比传统技术而言,利用石墨烯来进行DNA的测序,可以有效改善测序的速度、通量、可靠性以及准确率;研究者Dontschuk指出,每一个核酸碱基都会以一种明显不同的方式影响石墨烯的电子结构。当将石墨烯同纳米孔进行结合后,单一的DNA分子就可以穿过基于石墨烯的电子传感器,从而就会开启实时、高效及高通量的单一DNA分子的测序。

本文研究中研究者首次开展实验将石墨烯场效应管的原位电测量同光电发射光谱学技术进行结合来应用于实验中,对比了实验结果和同步加速器结果后,研究小组表示,他们就可以利用石墨烯装置来预测感知单分子的核酸碱基鸟嘌呤、胞嘧啶及胸腺嘧啶。

原始出处:

Nikolai Dontschuk,Alastair Stacey,Anton Tadich, Kevin J. Rietwyk,Alex Schenk, Mark T. Edmonds,Olga Shimoni,Chris I. Pakes,Steven Prawer& Jiri Cervenka.A graphene field-effect transistor as a molecule-specific probe of DNA nucleobases.Nature Communications, March 24, 2015; doi:10.1038/ncomms7563

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    2015-07-07 liuli5079
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    2015-12-23 liye789132251
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