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Clinica Chimica Acta：慢性髓系白血病患者血清伊马替尼及其代谢产物的测定

2019-11-23 gladiator MedSci原创

慢性髓系白血病患者血清
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伊马替尼具有良好的药代动力学特性，但随着时间的推移，初级和次级耐药机制可能导致临床反应的降低。血清伊马替尼浓度与治疗反应呈正相关。

伊马替尼具有良好的药代动力学特性，但随着时间的推移，初级和次级耐药机制可能导致临床反应的降低。血清伊马替尼浓度与治疗反应呈正相关。因此，本研究的目的是建立一种测定伊马替尼及其活性代谢物n -去甲基伊马替尼的方法。

采用液相色谱-串联质谱法(LC-MS/MS)测定血清伊马替尼和N-desmethyl imatinib水平，并根据CLSI (The Clinical & Laboratory Standards Institute)协议进行验证研究。收集40例慢性髓系白血病(CML)患者血清，采用LC-MS/MS和超高效液相色谱(UHPLC)方法进行分析。

LC-MS / MS方法的线性范围和相关系数分别为12.2–12,500 ng / mL和0.9987。 定量限为24.4μng/ mL。 伊马替尼和N-去甲基伊马替尼的保留时间分别为1.66和1.60 min。 两种方法的结果之间没有统计学上的差异。

这种LC-MS/MS方法具有成本效益和优点，如使用低血清量，仅需要简单的预处理步骤(仅蛋白沉淀)和分析的周转时间减少。

原始出处：

Duygu Eryavuz Onmaz，Sedat Abusoglu，Determination of serum imatinib and its' metabolite in patients chronic myeloid leukemia

本文系梅斯医学（MedSci）原创编译整理，转载需授权！

 

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    2020-07-12 windight

    #CTA#

    0

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    2019-12-30 丁鹏鹏

    #测定#

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    2020-04-21 fangcong

    #髓系白血病#

    0

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    2019-11-25 jmtang

    #慢性髓系白血病#

    0

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    2019-11-25 124987c6m39暂无昵称

    #代谢产物#

    0