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Mini-thermal platform integrated with microfluidic device with on-site detection for real-time DNA amplification

期刊: BIOTECHNIQUES, 2023; 74 (4)

The recent cases of COVID-19 have brought the prospect of and requirement for point-of-care diagnostic devices into the limelight. Despite all the adv......

Amberlite XAD-4 is a convenient tool for removing Triton X-100 and Sarkosyl from protein solutions

期刊: BIOTECHNIQUES, 2023; 74 (1)

Amberlite has been shown to be an appropriate material for the adsorption of organic contaminants from aqueous solutions. In addition, Amberlite XAD-2......

NANOTECHNOLOGY AND CANCER THERAPEUTICS: DELIVERING ON THE HYPE?

期刊: BIOTECHNIQUES, 2023; 74 (2)

Nanoparticle drug delivery systems are a promising development in cancer therapy for reducing toxicity and drug resistance, whilst improving precision......

MICROBIAL MARVELS: COULD MICROBES RESOLVE CLIMATE CHANGE?

期刊: BIOTECHNIQUES, 2023; 74 (6)

Our planet faces many challenges. Namely, the damage caused by human disruption of natural levels of carbon and nitrogen as a result of the use of fos......

Accurate, fast and cost-effective simultaneous detection of bacterial meningitis by qualitative PCR with high-resolution melting

期刊: BIOTECHNIQUES, 2023; ()

Neisseria meningitidis, Haemophilus influenzae and Streptococcus pneumoniae are leading causes of meningitis and acute invasive infections. PCR-based ......

Site-specific analysis of ribosomal 2'O-methylation by quantitative reverse transcription PCR under low deoxynucleotide triphosphate concentrations

期刊: BIOTECHNIQUES, 2023; 74 (5)

Ribose 2'O-methylation (Nm, ribomethylation) is the most abundant RNA modification present in rRNA. It has been shown that alterations in ribo-somal 2......

MSCProfiler: a single cell image processing workflow to investigate mesenchymal stem cell heterogeneity

期刊: BIOTECHNIQUES, 2023; 75 (5)

Single cell cytometry has demonstrated plausible immuno-heterogeneity of mesenchymal stem cells (MSCs) owing to their multivariate stromal origin. To ......

Specific N-terminal amino acids potentiate the periplasmic expression of single-chain variable fragments in Escherichia coli

期刊: BIOTECHNIQUES, 2023; 74 (2)

Single-chain variable fragments (ScFvs) are important in therapy, diagnosis and research because of their elevated antigen affinity and low immunogeni......

Modification of Grocott's staining procedure with heat treatment and oxidation by periodic acid for mucormycosis in tissue: a method to detect Mucor spp.

期刊: BIOTECHNIQUES, 2023; 74 (3)

The sensitivity of the Grocott-modified Gomori's methenamine-silver nitrate technique for the detection of fungi is sometimes low, especially for Muco......

A novel use of a needle-free injection system for improved nucleic acid delivery and expression in vivo

期刊: BIOTECHNIQUES, 2023; 75 (2)

Transfection, a nonviral method of nucleic acid delivery, often exhibits poor efficiency in vivo. The needle-based in vivo delivery of transfection re......

Peter and the Beanstalk: tackling the giant questions of soybean nodulation

期刊: BIOTECHNIQUES, 2023; ()

The following is an edited interview, carried out by the Journal Development Editor of BioTechniques, Ashling Cannon, with Peter Gresshoff (University......

Microbial forensics: what we've learned from Amerithrax and beyond

期刊: BIOTECHNIQUES, 2023; 75 (4)

The Amerithrax investigation into anthrax letter attacks in the USA forever changed the game in microbial forensics. Here we review the techniques use......

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