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One-step metal affinity purification of recombinant hFGF19 without using tags

期刊: PROTEIN EXPRESSION AND PURIFICATION, 2023; 201 ()

Human fibroblast growth factor 19 (hFGF19) belongs to the endocrine FGF19 superfamily and is considered a potential agent to treat severe or relapsing......

A rapid procedure to generate stably transfected HEK293 suspension cells for recombinant protein manufacturing: Yield improvements, bioreactor production and downstream processing

期刊: PROTEIN EXPRESSION AND PURIFICATION, 2023; 210 ()

The human cell line HEK293 is one of the preferred choices for manufacturing therapeutic proteins and viral vectors for human applications. Despite it......

Heterologous protein production using Psychrobacter sp. PAMC 21119 analyzed with a green fluorescent protein-based reporter system

期刊: PROTEIN EXPRESSION AND PURIFICATION, 2023; 212 ()

Insolubility and low expression are typical bottlenecks in the production of proteins for studying their function and structure using X-ray crystallog......

Biochemical and biophysical characterization of the RAS family small GTPase protein DiRAS3

期刊: PROTEIN EXPRESSION AND PURIFICATION, 2023; 212 ()

DiRAS3, also called ARHI, is a RAS (sub)family small GTPase protein that shares 50-60% sequence identity with H-, K-, and N-RAS, with substitutions in......

Heterologous expression of recombinant nattokinase in Escherichia coli BL21(DE3) and media optimization for overproduction of nattokinase using RSM

期刊: PROTEIN EXPRESSION AND PURIFICATION, 2023; 203 ()

Nattokinase, a serine protease, was discovered in Bacillus subtilis during the fermentation of a soybean byproduct. Nattokinase is essential for the l......

The choice of chromatographic resin for the purification of recombinant lysostaphin affects its activity

期刊: PROTEIN EXPRESSION AND PURIFICATION, 2023; 207 ()

Lysostaphin is a zinc-dependent endopeptidase that is effective against both antibiotic-sensitive and antibiotic -resistant strains of Staphylococcus ......

A simple method to purify intrinsically disordered proteins by adjusting trichloroacetic acid concentration

期刊: PROTEIN EXPRESSION AND PURIFICATION, 2023; 202 ()

Late embryogenic abundant proteins (LEA) are a group of proteins that accumulate during the desiccation phase of the seed and in response to water def......

Optimized high-yield preparation of alkaline-solubilizable crystalline inclusion of the Bacillus thuringiensis Cry4Aa δ-endotoxin expressed in Escherichia coli

期刊: PROTEIN EXPRESSION AND PURIFICATION, 2023; 210 ()

The native Cry4Aa 6-endotoxin produced exclusively in Bacillus thuringiensis during sporulation as a-130-kDa inactive protoxin is confined within the ......

High yield expression and purification of full-length Neurotensin with pyroglutamate modification

期刊: PROTEIN EXPRESSION AND PURIFICATION, 2023; 204 ()

Neurotensin (NT) is a 13-residue endogenous peptide found in mammals, with neurotransmission and hormonal roles in the central nervous system and gast......

Optimization of a medium composition for the heterologous production of Alcaligenes faecalis penicillin G acylase in Bacillus megaterium

期刊: PROTEIN EXPRESSION AND PURIFICATION, 2023; 210 ()

Penicillin G acylase (PGA) is a strategic enzyme in the production processes of beta-lactam antibiotics. High demand for & beta;-lactam semisynthe......

Column-free purification of an artificial protein nanocage, TIP60

期刊: PROTEIN EXPRESSION AND PURIFICATION, 2023; 205 ()

Protein nanocages, which have inner cavities and surface pores, are attractive materials for various applications, such as in catalysts and medicine. ......

Simple purification and characterization of soluble and homogenous ABC-F translation factors from Enterococcus faecium

期刊: PROTEIN EXPRESSION AND PURIFICATION, 2023; 210 ()

The family of ATP-binding cassette F proteins (ABC-F) is mainly made up of cytosolic proteins involved in regulating protein synthesis, and they are o......

Bioindustrial applications of thermostable Endoglucanase purified from Trichoderma viride towards the conversion of agrowastes to value-added products

期刊: PROTEIN EXPRESSION AND PURIFICATION, 2023; 211 ()

Importance of biocatalytic reactions and biotransformations mediated by fungal enzymes has increased tremendously in various industries. Endoglucanase......

A comprehensive method for modeling and simulating ion exchange chromatography of complex mixtures

期刊: PROTEIN EXPRESSION AND PURIFICATION, 2023; 205 ()

In recent years, many biological-based products have been developed, representing a significant fraction of income in the pharmaceutical market. Ion e......

Apolipoprotein E4 heterologous expression, purification under non-denaturing conditions, and effects on neuronal clonal cell lines

期刊: PROTEIN EXPRESSION AND PURIFICATION, 2023; 210 ()

The & epsilon;4 allele of the apolipoprotein E gene (APOE4) constitutes the main genetic risk factor for late-onset Alzheimer disease (AD). High a......

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