Phage-assisted continuous evolution of proteases with altered substrate specificity

Packer, MS; Rees, HA; Liu, DR

Liu, DR (reprint author), Harvard Univ, Dept Chem & Chem Biol, 12 Oxford St, Cambridge, MA 02138 USA.; Liu, DR (reprint author), Broad Inst MIT & Harvard, 75 Ames St, Cambridge, MA 02142 USA.; Liu, DR (reprint author), Harvard Univ, Howard Hughes Med Inst

NATURE COMMUNICATIONS, 2017; 8 ( ):

Abstract

Here we perform phage-assisted continuous evolution (PACE) of TEV protease, which canonically cleaves ENLYFQS, to cleave a very different target seque......

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