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Mol Med Rep：桃叶珊瑚可抑制钛颗粒诱导的细胞凋亡并促进成骨

2018-07-19 MedSci MedSci原创

桃叶珊瑚钛成骨
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桃叶珊瑚苷是从多种中草药中分离出的环烯醚萜苷，具有多种药理活性。本研究旨在探究桃叶珊瑚苷抑制钛颗粒诱导的小鼠MC3T3-E1成骨细胞凋亡和促进骨形成的作用和机制。使用MTT测定和流式细胞术分别分析细胞活力和细胞凋亡。使用ELISA和对硝基苯磷酸盐比色法评估氧化应激标记物和碱性磷酸酶活性（ALP）。Western印迹和逆转录-定量聚合酶链反应测定用于评估相关的mRNA和蛋白质表达。结果表明，桃叶珊瑚

桃叶珊瑚苷是从多种中草药中分离出的环烯醚萜苷，具有多种药理活性。本研究旨在探究桃叶珊瑚苷抑制钛颗粒诱导的小鼠MC3T3-E1成骨细胞凋亡和促进骨形成的作用和机制。

使用MTT测定和流式细胞术分别分析细胞活力和细胞凋亡。使用ELISA和对硝基苯磷酸盐比色法评估氧化应激标记物和碱性磷酸酶活性（ALP）。Western印迹和逆转录-定量聚合酶链反应测定用于评估相关的mRNA和蛋白质表达。

结果表明，桃叶珊瑚苷增强了Ti颗粒处理的MC3T3-E1细胞的细胞活性。桃叶珊瑚苷通过影响B细胞淋巴瘤-2（Bcl-2），Bcl-2相关X蛋白，ALP和相关的成骨因子表达来抑制Ti颗粒诱导的MC3T3-E1细胞的凋亡并促进成骨。桃叶珊瑚苷降低了Ti颗粒诱导的MC3T3-E1细胞中的氧化应激。此外，桃叶珊瑚苷在Ti颗粒诱导的MC3T3-E1细胞中上调骨形态发生蛋白2（BMP2）/Smads/runt相关转录因子2（RunX2）通路。

总之，本研究证实，桃叶珊瑚苷抑制了Ti颗粒介导的MC3T3-E1细胞凋亡，并通过影响BMP2/Smads/RunX2信号通路促进成骨。

原始出处：

Zhu Z, Xie Q, et al., Aucubin suppresses Titanium particles‑mediated apoptosis of MC3T3‑E1 cells and facilitates osteogenesis by affecting the BMP2/Smads/RunX2 signaling pathway. Mol Med Rep. 2018 Jul 16. doi: 10.3892/mmr.2018.9286.

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    2018-10-06 30397613

    #钛#

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    2018-07-21 ms8538335253054589

    #细胞凋亡#

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    2018-07-21 zhaojie88

    #成骨#

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    2018-07-21 ms3994565386320060

    #Med#

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    2018-07-19 1ddf0692m34(暂无匿称)

    学习了，长知识

    0