检验

订阅

MedSci主页 / 所有栏目 / 检验

Sci Rep：硼酸改性磁性纳米粒子可灵敏特异性检测羊水中的糖肽

2017-08-11 MedSci MedSci原创

硼酸纳米微球糖肽
关注

虽然介孔材料和磁性材料都可用于富集糖肽，但是联合介孔结构和磁材料用于糖肽分析的研究目前尚未有研究报道。本研究中，通过将硼酸分子共价结合到硅烷化Fe3O4@mTiO2微球表面，研究人员成功制备了硼酸改性的磁性Fe3O4@mTiO2。最终所获得的纳米粒子（B-Fe3O4@mTiO2）经检测显示存在典型的磁滞曲线，表明具有超顺磁行为；虽然粒子的表面积和孔体积下降，但是这些粒子的介孔尺寸并没有变化。通过将

虽然介孔材料和磁性材料都可用于富集糖肽，但是联合介孔结构和磁材料用于糖肽分析的研究目前尚未有研究报道。

本研究中，通过将硼酸分子共价结合到硅烷化Fe3O4@mTiO2微球表面，研究人员成功制备了硼酸改性的磁性Fe3O4@mTiO2。最终所获得的纳米粒子（B-Fe3O4@mTiO2）经检测显示存在典型的磁滞曲线，表明具有超顺磁行为；虽然粒子的表面积和孔体积下降，但是这些粒子的介孔尺寸并没有变化。

通过将这些粒子与传统的聚（甲基丙烯酸甲酯）（PMMA）微球结合，研究人员开发出可灵敏且特异性检测糖肽/糖蛋白的协同方法。由于PMMA微球的引入，可吸附并富集糖肽/糖蛋白，无论是检测的糖肽数目还是标准蛋白混合物信噪比都得到明显的提高。通过此协同方法糖肽的回收率达到92.1%，而B-Fe3O4@mTiO2单独应用时回收率为75.3%。最后，研究人员测试了这种方法在羊水内富集并检测糖肽的效果，结果检测到的糖肽的最大数目和比例均较单独应用B-Fe3O4@mTiO2及商业化的SiMAG-硼酸颗粒高。

总之，该研究结果表明，这种联合B-Fe3O4@mTiO2和PMMA微球的协同方法为富集糖蛋白提供了一种新的检测方法。

原始出处：

Zhonghua Shi, Liyong Pu, et al., Boronic Acid-Modified Magnetic Fe3O4@mTiO2 Microspheres for Highly Sensitive and Selective Enrichment of N-Glycopeptides in Amniotic Fluid. Sci Rep. 2017; 7: 4603. Published online 2017 Jul 4. doi: 10.1038/s41598-017-04517-8.

版权声明：
本网站所有内容来源注明为“梅斯医学”或“MedSci原创”的文字、图片和音视频资料，版权均属于梅斯医学所有。非经授权，任何媒体、网站或个人不得转载，授权转载时须注明来源为“梅斯医学”。其它来源的文章系转载文章，或“梅斯号”自媒体发布的文章，仅系出于传递更多信息之目的，本站仅负责审核内容合规，其内容不代表本站立场，本站不负责内容的准确性和版权。如果存在侵权、或不希望被转载的媒体或个人可与我们联系，我们将立即进行删除处理。
在此留言
评论区 (7)
#插入话题
  1. [GetPortalCommentsPageByObjectIdResponse(id=1899150, encodeId=58b51899150bf, content=<a href='/topic/show?id=6f746e94170' target=_blank style='color:#2F92EE;'>#特异性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67941, encryptionId=6f746e94170, topicName=特异性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=dc7a157, createdName=jinweidong, createdTime=Mon Oct 16 02:58:00 CST 2017, time=2017-10-16, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1667981, encodeId=33d4166e981c9, content=<a href='/topic/show?id=1e12e9674b8' target=_blank style='color:#2F92EE;'>#羊水#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=37, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=79674, encryptionId=1e12e9674b8, topicName=羊水)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=915326217791, createdName=陈吴1243, createdTime=Thu Jun 14 02:58:00 CST 2018, time=2018-06-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1801406, encodeId=ee1c180140605, content=<a href='/topic/show?id=cccfe3791bd' target=_blank style='color:#2F92EE;'>#磁性纳米#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=73791, encryptionId=cccfe3791bd, topicName=磁性纳米)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6cc1239, createdName=sodoo, createdTime=Mon Mar 05 16:58:00 CST 2018, time=2018-03-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1772935, encodeId=e3201e72935e6, content=<a href='/topic/show?id=89f3e742947' target=_blank style='color:#2F92EE;'>#纳米粒#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77429, encryptionId=89f3e742947, topicName=纳米粒)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=e8ae38449394, createdName=zjubiostat, createdTime=Wed Jul 11 15:58:00 CST 2018, time=2018-07-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=233896, encodeId=1d9a23389636, content=学习了, beContent=null, objectType=article, channel=null, level=null, likeNumber=42, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=c3da1990174, createdName=yfjms, createdTime=Tue Aug 15 22:18:51 CST 2017, time=2017-08-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1580614, encodeId=7d9915806145d, content=<a href='/topic/show?id=d4c6e7430be' target=_blank style='color:#2F92EE;'>#纳米粒子#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=35, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77430, encryptionId=d4c6e7430be, topicName=纳米粒子)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=eedc16636878, createdName=ms920641618706883, createdTime=Sun Aug 13 07:58:00 CST 2017, time=2017-08-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=232887, encodeId=fe6523288e83, content=学习，谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=43, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/aLGWoFXAyMbIu3qymFOyhb9xDeHojCicsdRdyC3XXdGk2ib9yLypZuHN06WeW4HO8WN0GGrgEfvnTX8gsckof05GHOU5OM4Mvx/0, createdBy=9cee1982656, createdName=执着追梦, createdTime=Sat Aug 12 14:20:55 CST 2017, time=2017-08-12, status=1, ipAttribution=)]
    2017-10-16 jinweidong

    #特异性#

    0

  2. [GetPortalCommentsPageByObjectIdResponse(id=1899150, encodeId=58b51899150bf, content=<a href='/topic/show?id=6f746e94170' target=_blank style='color:#2F92EE;'>#特异性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67941, encryptionId=6f746e94170, topicName=特异性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=dc7a157, createdName=jinweidong, createdTime=Mon Oct 16 02:58:00 CST 2017, time=2017-10-16, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1667981, encodeId=33d4166e981c9, content=<a href='/topic/show?id=1e12e9674b8' target=_blank style='color:#2F92EE;'>#羊水#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=37, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=79674, encryptionId=1e12e9674b8, topicName=羊水)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=915326217791, createdName=陈吴1243, createdTime=Thu Jun 14 02:58:00 CST 2018, time=2018-06-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1801406, encodeId=ee1c180140605, content=<a href='/topic/show?id=cccfe3791bd' target=_blank style='color:#2F92EE;'>#磁性纳米#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=73791, encryptionId=cccfe3791bd, topicName=磁性纳米)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6cc1239, createdName=sodoo, createdTime=Mon Mar 05 16:58:00 CST 2018, time=2018-03-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1772935, encodeId=e3201e72935e6, content=<a href='/topic/show?id=89f3e742947' target=_blank style='color:#2F92EE;'>#纳米粒#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77429, encryptionId=89f3e742947, topicName=纳米粒)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=e8ae38449394, createdName=zjubiostat, createdTime=Wed Jul 11 15:58:00 CST 2018, time=2018-07-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=233896, encodeId=1d9a23389636, content=学习了, beContent=null, objectType=article, channel=null, level=null, likeNumber=42, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=c3da1990174, createdName=yfjms, createdTime=Tue Aug 15 22:18:51 CST 2017, time=2017-08-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1580614, encodeId=7d9915806145d, content=<a href='/topic/show?id=d4c6e7430be' target=_blank style='color:#2F92EE;'>#纳米粒子#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=35, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77430, encryptionId=d4c6e7430be, topicName=纳米粒子)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=eedc16636878, createdName=ms920641618706883, createdTime=Sun Aug 13 07:58:00 CST 2017, time=2017-08-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=232887, encodeId=fe6523288e83, content=学习，谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=43, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/aLGWoFXAyMbIu3qymFOyhb9xDeHojCicsdRdyC3XXdGk2ib9yLypZuHN06WeW4HO8WN0GGrgEfvnTX8gsckof05GHOU5OM4Mvx/0, createdBy=9cee1982656, createdName=执着追梦, createdTime=Sat Aug 12 14:20:55 CST 2017, time=2017-08-12, status=1, ipAttribution=)]
    2018-06-14 陈吴1243

    #羊水#

    0

  3. [GetPortalCommentsPageByObjectIdResponse(id=1899150, encodeId=58b51899150bf, content=<a href='/topic/show?id=6f746e94170' target=_blank style='color:#2F92EE;'>#特异性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67941, encryptionId=6f746e94170, topicName=特异性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=dc7a157, createdName=jinweidong, createdTime=Mon Oct 16 02:58:00 CST 2017, time=2017-10-16, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1667981, encodeId=33d4166e981c9, content=<a href='/topic/show?id=1e12e9674b8' target=_blank style='color:#2F92EE;'>#羊水#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=37, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=79674, encryptionId=1e12e9674b8, topicName=羊水)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=915326217791, createdName=陈吴1243, createdTime=Thu Jun 14 02:58:00 CST 2018, time=2018-06-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1801406, encodeId=ee1c180140605, content=<a href='/topic/show?id=cccfe3791bd' target=_blank style='color:#2F92EE;'>#磁性纳米#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=73791, encryptionId=cccfe3791bd, topicName=磁性纳米)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6cc1239, createdName=sodoo, createdTime=Mon Mar 05 16:58:00 CST 2018, time=2018-03-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1772935, encodeId=e3201e72935e6, content=<a href='/topic/show?id=89f3e742947' target=_blank style='color:#2F92EE;'>#纳米粒#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77429, encryptionId=89f3e742947, topicName=纳米粒)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=e8ae38449394, createdName=zjubiostat, createdTime=Wed Jul 11 15:58:00 CST 2018, time=2018-07-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=233896, encodeId=1d9a23389636, content=学习了, beContent=null, objectType=article, channel=null, level=null, likeNumber=42, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=c3da1990174, createdName=yfjms, createdTime=Tue Aug 15 22:18:51 CST 2017, time=2017-08-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1580614, encodeId=7d9915806145d, content=<a href='/topic/show?id=d4c6e7430be' target=_blank style='color:#2F92EE;'>#纳米粒子#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=35, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77430, encryptionId=d4c6e7430be, topicName=纳米粒子)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=eedc16636878, createdName=ms920641618706883, createdTime=Sun Aug 13 07:58:00 CST 2017, time=2017-08-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=232887, encodeId=fe6523288e83, content=学习，谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=43, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/aLGWoFXAyMbIu3qymFOyhb9xDeHojCicsdRdyC3XXdGk2ib9yLypZuHN06WeW4HO8WN0GGrgEfvnTX8gsckof05GHOU5OM4Mvx/0, createdBy=9cee1982656, createdName=执着追梦, createdTime=Sat Aug 12 14:20:55 CST 2017, time=2017-08-12, status=1, ipAttribution=)]
    2018-03-05 sodoo

    #磁性纳米#

    0

  4. [GetPortalCommentsPageByObjectIdResponse(id=1899150, encodeId=58b51899150bf, content=<a href='/topic/show?id=6f746e94170' target=_blank style='color:#2F92EE;'>#特异性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67941, encryptionId=6f746e94170, topicName=特异性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=dc7a157, createdName=jinweidong, createdTime=Mon Oct 16 02:58:00 CST 2017, time=2017-10-16, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1667981, encodeId=33d4166e981c9, content=<a href='/topic/show?id=1e12e9674b8' target=_blank style='color:#2F92EE;'>#羊水#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=37, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=79674, encryptionId=1e12e9674b8, topicName=羊水)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=915326217791, createdName=陈吴1243, createdTime=Thu Jun 14 02:58:00 CST 2018, time=2018-06-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1801406, encodeId=ee1c180140605, content=<a href='/topic/show?id=cccfe3791bd' target=_blank style='color:#2F92EE;'>#磁性纳米#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=73791, encryptionId=cccfe3791bd, topicName=磁性纳米)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6cc1239, createdName=sodoo, createdTime=Mon Mar 05 16:58:00 CST 2018, time=2018-03-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1772935, encodeId=e3201e72935e6, content=<a href='/topic/show?id=89f3e742947' target=_blank style='color:#2F92EE;'>#纳米粒#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77429, encryptionId=89f3e742947, topicName=纳米粒)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=e8ae38449394, createdName=zjubiostat, createdTime=Wed Jul 11 15:58:00 CST 2018, time=2018-07-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=233896, encodeId=1d9a23389636, content=学习了, beContent=null, objectType=article, channel=null, level=null, likeNumber=42, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=c3da1990174, createdName=yfjms, createdTime=Tue Aug 15 22:18:51 CST 2017, time=2017-08-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1580614, encodeId=7d9915806145d, content=<a href='/topic/show?id=d4c6e7430be' target=_blank style='color:#2F92EE;'>#纳米粒子#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=35, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77430, encryptionId=d4c6e7430be, topicName=纳米粒子)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=eedc16636878, createdName=ms920641618706883, createdTime=Sun Aug 13 07:58:00 CST 2017, time=2017-08-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=232887, encodeId=fe6523288e83, content=学习，谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=43, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/aLGWoFXAyMbIu3qymFOyhb9xDeHojCicsdRdyC3XXdGk2ib9yLypZuHN06WeW4HO8WN0GGrgEfvnTX8gsckof05GHOU5OM4Mvx/0, createdBy=9cee1982656, createdName=执着追梦, createdTime=Sat Aug 12 14:20:55 CST 2017, time=2017-08-12, status=1, ipAttribution=)]
    2018-07-11 zjubiostat

    #纳米粒#

    0

  5. [GetPortalCommentsPageByObjectIdResponse(id=1899150, encodeId=58b51899150bf, content=<a href='/topic/show?id=6f746e94170' target=_blank style='color:#2F92EE;'>#特异性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67941, encryptionId=6f746e94170, topicName=特异性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=dc7a157, createdName=jinweidong, createdTime=Mon Oct 16 02:58:00 CST 2017, time=2017-10-16, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1667981, encodeId=33d4166e981c9, content=<a href='/topic/show?id=1e12e9674b8' target=_blank style='color:#2F92EE;'>#羊水#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=37, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=79674, encryptionId=1e12e9674b8, topicName=羊水)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=915326217791, createdName=陈吴1243, createdTime=Thu Jun 14 02:58:00 CST 2018, time=2018-06-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1801406, encodeId=ee1c180140605, content=<a href='/topic/show?id=cccfe3791bd' target=_blank style='color:#2F92EE;'>#磁性纳米#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=73791, encryptionId=cccfe3791bd, topicName=磁性纳米)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6cc1239, createdName=sodoo, createdTime=Mon Mar 05 16:58:00 CST 2018, time=2018-03-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1772935, encodeId=e3201e72935e6, content=<a href='/topic/show?id=89f3e742947' target=_blank style='color:#2F92EE;'>#纳米粒#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77429, encryptionId=89f3e742947, topicName=纳米粒)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=e8ae38449394, createdName=zjubiostat, createdTime=Wed Jul 11 15:58:00 CST 2018, time=2018-07-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=233896, encodeId=1d9a23389636, content=学习了, beContent=null, objectType=article, channel=null, level=null, likeNumber=42, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=c3da1990174, createdName=yfjms, createdTime=Tue Aug 15 22:18:51 CST 2017, time=2017-08-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1580614, encodeId=7d9915806145d, content=<a href='/topic/show?id=d4c6e7430be' target=_blank style='color:#2F92EE;'>#纳米粒子#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=35, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77430, encryptionId=d4c6e7430be, topicName=纳米粒子)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=eedc16636878, createdName=ms920641618706883, createdTime=Sun Aug 13 07:58:00 CST 2017, time=2017-08-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=232887, encodeId=fe6523288e83, content=学习，谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=43, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/aLGWoFXAyMbIu3qymFOyhb9xDeHojCicsdRdyC3XXdGk2ib9yLypZuHN06WeW4HO8WN0GGrgEfvnTX8gsckof05GHOU5OM4Mvx/0, createdBy=9cee1982656, createdName=执着追梦, createdTime=Sat Aug 12 14:20:55 CST 2017, time=2017-08-12, status=1, ipAttribution=)]
    2017-08-15 yfjms

    学习了

    0

  6. [GetPortalCommentsPageByObjectIdResponse(id=1899150, encodeId=58b51899150bf, content=<a href='/topic/show?id=6f746e94170' target=_blank style='color:#2F92EE;'>#特异性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67941, encryptionId=6f746e94170, topicName=特异性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=dc7a157, createdName=jinweidong, createdTime=Mon Oct 16 02:58:00 CST 2017, time=2017-10-16, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1667981, encodeId=33d4166e981c9, content=<a href='/topic/show?id=1e12e9674b8' target=_blank style='color:#2F92EE;'>#羊水#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=37, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=79674, encryptionId=1e12e9674b8, topicName=羊水)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=915326217791, createdName=陈吴1243, createdTime=Thu Jun 14 02:58:00 CST 2018, time=2018-06-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1801406, encodeId=ee1c180140605, content=<a href='/topic/show?id=cccfe3791bd' target=_blank style='color:#2F92EE;'>#磁性纳米#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=73791, encryptionId=cccfe3791bd, topicName=磁性纳米)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6cc1239, createdName=sodoo, createdTime=Mon Mar 05 16:58:00 CST 2018, time=2018-03-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1772935, encodeId=e3201e72935e6, content=<a href='/topic/show?id=89f3e742947' target=_blank style='color:#2F92EE;'>#纳米粒#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77429, encryptionId=89f3e742947, topicName=纳米粒)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=e8ae38449394, createdName=zjubiostat, createdTime=Wed Jul 11 15:58:00 CST 2018, time=2018-07-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=233896, encodeId=1d9a23389636, content=学习了, beContent=null, objectType=article, channel=null, level=null, likeNumber=42, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=c3da1990174, createdName=yfjms, createdTime=Tue Aug 15 22:18:51 CST 2017, time=2017-08-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1580614, encodeId=7d9915806145d, content=<a href='/topic/show?id=d4c6e7430be' target=_blank style='color:#2F92EE;'>#纳米粒子#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=35, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77430, encryptionId=d4c6e7430be, topicName=纳米粒子)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=eedc16636878, createdName=ms920641618706883, createdTime=Sun Aug 13 07:58:00 CST 2017, time=2017-08-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=232887, encodeId=fe6523288e83, content=学习，谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=43, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/aLGWoFXAyMbIu3qymFOyhb9xDeHojCicsdRdyC3XXdGk2ib9yLypZuHN06WeW4HO8WN0GGrgEfvnTX8gsckof05GHOU5OM4Mvx/0, createdBy=9cee1982656, createdName=执着追梦, createdTime=Sat Aug 12 14:20:55 CST 2017, time=2017-08-12, status=1, ipAttribution=)]
    2017-08-13 ms920641618706883

    #纳米粒子#

    0

  7. [GetPortalCommentsPageByObjectIdResponse(id=1899150, encodeId=58b51899150bf, content=<a href='/topic/show?id=6f746e94170' target=_blank style='color:#2F92EE;'>#特异性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67941, encryptionId=6f746e94170, topicName=特异性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=dc7a157, createdName=jinweidong, createdTime=Mon Oct 16 02:58:00 CST 2017, time=2017-10-16, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1667981, encodeId=33d4166e981c9, content=<a href='/topic/show?id=1e12e9674b8' target=_blank style='color:#2F92EE;'>#羊水#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=37, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=79674, encryptionId=1e12e9674b8, topicName=羊水)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=915326217791, createdName=陈吴1243, createdTime=Thu Jun 14 02:58:00 CST 2018, time=2018-06-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1801406, encodeId=ee1c180140605, content=<a href='/topic/show?id=cccfe3791bd' target=_blank style='color:#2F92EE;'>#磁性纳米#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=73791, encryptionId=cccfe3791bd, topicName=磁性纳米)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6cc1239, createdName=sodoo, createdTime=Mon Mar 05 16:58:00 CST 2018, time=2018-03-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1772935, encodeId=e3201e72935e6, content=<a href='/topic/show?id=89f3e742947' target=_blank style='color:#2F92EE;'>#纳米粒#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77429, encryptionId=89f3e742947, topicName=纳米粒)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=e8ae38449394, createdName=zjubiostat, createdTime=Wed Jul 11 15:58:00 CST 2018, time=2018-07-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=233896, encodeId=1d9a23389636, content=学习了, beContent=null, objectType=article, channel=null, level=null, likeNumber=42, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=c3da1990174, createdName=yfjms, createdTime=Tue Aug 15 22:18:51 CST 2017, time=2017-08-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1580614, encodeId=7d9915806145d, content=<a href='/topic/show?id=d4c6e7430be' target=_blank style='color:#2F92EE;'>#纳米粒子#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=35, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77430, encryptionId=d4c6e7430be, topicName=纳米粒子)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=eedc16636878, createdName=ms920641618706883, createdTime=Sun Aug 13 07:58:00 CST 2017, time=2017-08-13, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=232887, encodeId=fe6523288e83, content=学习，谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=43, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/aLGWoFXAyMbIu3qymFOyhb9xDeHojCicsdRdyC3XXdGk2ib9yLypZuHN06WeW4HO8WN0GGrgEfvnTX8gsckof05GHOU5OM4Mvx/0, createdBy=9cee1982656, createdName=执着追梦, createdTime=Sat Aug 12 14:20:55 CST 2017, time=2017-08-12, status=1, ipAttribution=)]
    2017-08-12 执着追梦

    学习，谢谢分享

    0