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J Biomed Mater Res A：重组人BMP-2与BMP-2质粒DNA的骨再生行为的比较分析

2018-11-01 MedSci MedSci原创

关注

骨再生和植入物的骨诱导能力是临床医学中的挑战性问题。目前，重组生长因子和非病毒基因转移是最常研究的骨生长增强方法，尽管更有利的方法仍不清楚。文献中缺乏关于这些骨再生方法的体内比较的知识。BMP-2是最常用的成骨生长因子，以最有效的剂量作为重组生长因子（rhBMP-2）和作为生长因子编码共聚物保护的基因载体（pBMP-2）应用于临床尺寸骨缺损（CSD）模型，以确定最适合骨再生的方法。 在32只

骨再生和植入物的骨诱导能力是临床医学中的挑战性问题。目前，重组生长因子和非病毒基因转移是最常研究的骨生长增强方法，尽管更有利的方法仍不清楚。文献中缺乏关于这些骨再生方法的体内比较的知识。BMP-2是最常用的成骨生长因子，以最有效的剂量作为重组生长因子（rhBMP-2）和作为生长因子编码共聚物保护的基因载体（pBMP-2）应用于临床尺寸骨缺损（CSD）模型，以确定最适合骨再生的方法。

在32只Sprague-Dawley大鼠中双侧诱导CSDs。将RhBMP-2（62.5μg）或pBMP-2（2.5μg）包埋在聚（d，l-）丙交酯涂覆的钛盘中。培养时间设定为14天，28天，56天和112天。安乐死后，通过微型计算机断层扫描，多色连续荧光标记和免疫组织化学分析样品。

结果显示，尽管两组中的骨缺陷在56天后与新骨桥接，但rhBMP-2最初诱导异位新骨形成，其随后以无组织的低密度方式重塑。相反，pBMP-2导致具有生理组织形态的较慢但稳定的骨再生，如通过骨钙蛋白染色显示的高成骨细胞活性所证实的。CD68和TRAP染色证实了rhBMP-2组破骨细胞活性较高。pBMP-2成功诱导局部控制的生理性骨再生，而rhBMP-2触发快速和异位骨形成，但骨形成不足。因此，非病毒基因转移似乎更有利于临床应用。

原始出处：

Kolk A, Boskov M, et al., Comparative analysis of bone regeneration behavior using recombinant human BMP-2 versus plasmid DNA of BMP-2. J Biomed Mater Res A. 2018 Oct 25. doi: 10.1002/jbm.a.36545. 

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    2019-04-12 江川靖瑶

    #质粒DNA#

    0

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    2019-01-14 nakerunner

    #比较分析#

    0

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    2019-01-14 12498f17m73暂无昵称

    #BMP-2#

    0

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    2018-12-03 sunylz

    #Bio#

    0

  5. [GetPortalCommentsPageByObjectIdResponse(id=1813392, encodeId=d4e0181339258, content=<a href='/topic/show?id=44de923e31b' target=_blank style='color:#2F92EE;'>#质粒DNA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=24, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=92373, encryptionId=44de923e31b, topicName=质粒DNA)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTK17mLkWpia3T92NlEWTkEhrhZa7XPNic2JqQumEicZXGYFG6Ww5SCIl0Uq62bhhPnibAqIZDHtRva92A/132, createdBy=e0752500025, createdName=江川靖瑶, createdTime=Fri Apr 12 02:57:00 CST 2019, time=2019-04-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1988394, encodeId=64ac19883942d, content=<a href='/topic/show?id=18ae6365278' target=_blank style='color:#2F92EE;'>#比较分析#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=37, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=63652, encryptionId=18ae6365278, topicName=比较分析)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=0cd7162, createdName=nakerunner, createdTime=Mon Jan 14 12:57:00 CST 2019, time=2019-01-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1642662, encodeId=3e35164266216, content=<a href='/topic/show?id=814c35e6e2' target=_blank style='color:#2F92EE;'>#BMP-2#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=34, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3576, encryptionId=814c35e6e2, topicName=BMP-2)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=120c22999408, createdName=12498f17m73暂无昵称, createdTime=Mon Jan 14 06:57:00 CST 2019, time=2019-01-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1918372, encodeId=ad8e19183e2e0, content=<a href='/topic/show?id=4f26341994' target=_blank style='color:#2F92EE;'>#Bio#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3419, encryptionId=4f26341994, topicName=Bio)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=12de429, createdName=sunylz, createdTime=Mon Dec 03 05:57:00 CST 2018, time=2018-12-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1634053, encodeId=22c1163405380, content=<a href='/topic/show?id=596635e549' target=_blank style='color:#2F92EE;'>#BMP#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3575, encryptionId=596635e549, topicName=BMP)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=d95322064897, createdName=HNYYM, createdTime=Thu Jan 03 23:57:00 CST 2019, time=2019-01-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1472160, encodeId=cf1214e2160d9, content=<a href='/topic/show?id=d068101610b9' target=_blank style='color:#2F92EE;'>#骨再生#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=101610, encryptionId=d068101610b9, topicName=骨再生)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=a4cf7208806, createdName=lsj630, createdTime=Sat Nov 03 13:57:00 CST 2018, time=2018-11-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1609282, encodeId=819016092823c, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=24, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Sat Nov 03 13:57:00 CST 2018, time=2018-11-03, status=1, ipAttribution=)]
    2019-01-03 HNYYM

    #BMP#

    0

  6. [GetPortalCommentsPageByObjectIdResponse(id=1813392, encodeId=d4e0181339258, content=<a href='/topic/show?id=44de923e31b' target=_blank style='color:#2F92EE;'>#质粒DNA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=24, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=92373, encryptionId=44de923e31b, topicName=质粒DNA)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTK17mLkWpia3T92NlEWTkEhrhZa7XPNic2JqQumEicZXGYFG6Ww5SCIl0Uq62bhhPnibAqIZDHtRva92A/132, createdBy=e0752500025, createdName=江川靖瑶, createdTime=Fri Apr 12 02:57:00 CST 2019, time=2019-04-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1988394, encodeId=64ac19883942d, content=<a href='/topic/show?id=18ae6365278' target=_blank style='color:#2F92EE;'>#比较分析#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=37, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=63652, encryptionId=18ae6365278, topicName=比较分析)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=0cd7162, createdName=nakerunner, createdTime=Mon Jan 14 12:57:00 CST 2019, time=2019-01-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1642662, encodeId=3e35164266216, content=<a href='/topic/show?id=814c35e6e2' target=_blank style='color:#2F92EE;'>#BMP-2#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=34, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3576, encryptionId=814c35e6e2, topicName=BMP-2)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=120c22999408, createdName=12498f17m73暂无昵称, createdTime=Mon Jan 14 06:57:00 CST 2019, time=2019-01-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1918372, encodeId=ad8e19183e2e0, content=<a href='/topic/show?id=4f26341994' target=_blank style='color:#2F92EE;'>#Bio#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3419, encryptionId=4f26341994, topicName=Bio)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=12de429, createdName=sunylz, createdTime=Mon Dec 03 05:57:00 CST 2018, time=2018-12-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1634053, encodeId=22c1163405380, content=<a href='/topic/show?id=596635e549' target=_blank style='color:#2F92EE;'>#BMP#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3575, encryptionId=596635e549, topicName=BMP)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=d95322064897, createdName=HNYYM, createdTime=Thu Jan 03 23:57:00 CST 2019, time=2019-01-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1472160, encodeId=cf1214e2160d9, content=<a href='/topic/show?id=d068101610b9' target=_blank style='color:#2F92EE;'>#骨再生#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=101610, encryptionId=d068101610b9, topicName=骨再生)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=a4cf7208806, createdName=lsj630, createdTime=Sat Nov 03 13:57:00 CST 2018, time=2018-11-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1609282, encodeId=819016092823c, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=24, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Sat Nov 03 13:57:00 CST 2018, time=2018-11-03, status=1, ipAttribution=)]
    2018-11-03 lsj630

    #骨再生#

    0

  7. [GetPortalCommentsPageByObjectIdResponse(id=1813392, encodeId=d4e0181339258, content=<a href='/topic/show?id=44de923e31b' target=_blank style='color:#2F92EE;'>#质粒DNA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=24, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=92373, encryptionId=44de923e31b, topicName=质粒DNA)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTK17mLkWpia3T92NlEWTkEhrhZa7XPNic2JqQumEicZXGYFG6Ww5SCIl0Uq62bhhPnibAqIZDHtRva92A/132, createdBy=e0752500025, createdName=江川靖瑶, createdTime=Fri Apr 12 02:57:00 CST 2019, time=2019-04-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1988394, encodeId=64ac19883942d, content=<a href='/topic/show?id=18ae6365278' target=_blank style='color:#2F92EE;'>#比较分析#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=37, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=63652, encryptionId=18ae6365278, topicName=比较分析)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=0cd7162, createdName=nakerunner, createdTime=Mon Jan 14 12:57:00 CST 2019, time=2019-01-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1642662, encodeId=3e35164266216, content=<a href='/topic/show?id=814c35e6e2' target=_blank style='color:#2F92EE;'>#BMP-2#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=34, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3576, encryptionId=814c35e6e2, topicName=BMP-2)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=120c22999408, createdName=12498f17m73暂无昵称, createdTime=Mon Jan 14 06:57:00 CST 2019, time=2019-01-14, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1918372, encodeId=ad8e19183e2e0, content=<a href='/topic/show?id=4f26341994' target=_blank style='color:#2F92EE;'>#Bio#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3419, encryptionId=4f26341994, topicName=Bio)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=12de429, createdName=sunylz, createdTime=Mon Dec 03 05:57:00 CST 2018, time=2018-12-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1634053, encodeId=22c1163405380, content=<a href='/topic/show?id=596635e549' target=_blank style='color:#2F92EE;'>#BMP#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3575, encryptionId=596635e549, topicName=BMP)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=d95322064897, createdName=HNYYM, createdTime=Thu Jan 03 23:57:00 CST 2019, time=2019-01-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1472160, encodeId=cf1214e2160d9, content=<a href='/topic/show?id=d068101610b9' target=_blank style='color:#2F92EE;'>#骨再生#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=101610, encryptionId=d068101610b9, topicName=骨再生)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=a4cf7208806, createdName=lsj630, createdTime=Sat Nov 03 13:57:00 CST 2018, time=2018-11-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1609282, encodeId=819016092823c, content=<a href='/topic/show?id=eb6b1145939' target=_blank style='color:#2F92EE;'>#Med#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=24, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11459, encryptionId=eb6b1145939, topicName=Med)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=552a19396740, createdName=ms3994565386320060, createdTime=Sat Nov 03 13:57:00 CST 2018, time=2018-11-03, status=1, ipAttribution=)]
    2018-11-03 ms3994565386320060

    #Med#

    0