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Acta Biomater：外泌体整合的氧化钛纳米管用于靶向骨再生

2019-01-26 MedSci MedSci原创

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的调节作用通过整合来自BMP2刺激的巨噬细胞的外来体来促进支架的功能化，以避免异位骨形成并减少副作用。为了确定BMP2刺激后来自巨噬细胞的外来体纳米载体的功能，我们从Dulbecco改良的Eagle培养基（DMEM）或BMP2刺激的巨噬细胞中分离外泌体，并评估它们对成骨的影响。结果显示，来自DMEM-或BMP2-处理的巨噬细胞的外来体的形态学表征显示没有明显差异，并且骨髓来源的间充质基质细胞显示两

的调节作用通过整合来自BMP2刺激的巨噬细胞的外来体来促进支架的功能化，以避免异位骨形成并减少副作用。为了确定BMP2刺激后来自巨噬细胞的外来体纳米载体的功能，我们从Dulbecco改良的Eagle培养基（DMEM）或BMP2刺激的巨噬细胞中分离外泌体，并评估它们对成骨的影响。

结果显示，来自DMEM-或BMP2-处理的巨噬细胞的外来体的形态学表征显示没有明显差异，并且骨髓来源的间充质基质细胞显示两种外来体的相似细胞摄取模式。使用BMP2 /巨噬细胞衍生的外泌体的体外研究表明它们对成骨分化的有益作用。为了改善钛植入物的生物功能性，使用BMP2 /巨噬细胞衍生的外泌体来修饰钛纳米管植入物以促进骨生成。BMP2 /巨噬细胞衍生的外泌体的掺入明显增加早期成骨细胞分化标志物，碱性磷酸酶（ALP）和BMP2的表达，表明掺入BMP2 /巨噬细胞衍生的外泌体的钛纳米管的促成骨作用。用BMP2 /巨噬细胞衍生的外泌体功能化的钛纳米管在成骨分化期间激活自噬。

总之，外泌体整合的钛纳米管可以作为骨再生的新兴功能材料。本研究的贡献包括（1）证明BMP2 /巨噬细胞衍生的外泌体对间充质基质细胞（MSCs）成骨分化的潜在调节作用; （2）掺入外泌体的钛纳米管的制造; （3）对用于安全使用BMP2的钛纳米管材料的应用提供了新见解。

原始出处：

Wei F, Li M, et al., Exosome-integrated titanium oxide nanotubes for targeted bone regeneration. Acta Biomater. 2019 Jan 7. pii: S1742-7061(19)30017-0. doi: 10.1016/j.actbio.2019.01.006.

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    2019-10-10 sunylz

    #Bio#

    0

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    2020-01-01 30397613

    #钛#

    0

  3. [GetPortalCommentsPageByObjectIdResponse(id=1918500, encodeId=4ca4191850046, content=<a href='/topic/show?id=4f26341994' target=_blank style='color:#2F92EE;'>#Bio#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=22, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3419, encryptionId=4f26341994, topicName=Bio)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=12de429, createdName=sunylz, createdTime=Thu Oct 10 14:01:00 CST 2019, time=2019-10-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1679757, encodeId=014f16e975724, content=<a href='/topic/show?id=d4ac9639efa' target=_blank style='color:#2F92EE;'>#钛#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=24, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=96397, encryptionId=d4ac9639efa, topicName=钛)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=927227807332, createdName=30397613, createdTime=Wed Jan 01 22:01:00 CST 2020, time=2020-01-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1849632, encodeId=f102184963259, content=<a href='/topic/show?id=61815385aa' target=_blank style='color:#2F92EE;'>#CTA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=5385, encryptionId=61815385aa, topicName=CTA)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=ec9955, createdName=windight, createdTime=Fri Nov 08 20:01:00 CST 2019, time=2019-11-08, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1472169, encodeId=4fca14e21691f, content=<a href='/topic/show?id=d068101610b9' target=_blank style='color:#2F92EE;'>#骨再生#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=101610, encryptionId=d068101610b9, topicName=骨再生)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=a4cf7208806, createdName=lsj630, createdTime=Mon Jan 28 06:01:00 CST 2019, time=2019-01-28, status=1, ipAttribution=)]
    2019-11-08 windight

    #CTA#

    0

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    2019-01-28 lsj630

    #骨再生#

    0