新型电化学传感器可灵敏检测体液中尿酸及叶酸的浓度

2017-08-10 MedSci MedSci原创

尿酸(UA)和叶酸(FA)一直是生物医学所关注的化合物。在人类中,大约70%的每日尿酸通过肾脏产生,5- 25%的人由于肾排泄障碍导致高尿酸血症。众所周知,叶酸属于B族维生素,女性常补充以防止孕期神经管缺陷的发生。本研究中,研究人员使用新型两步法合成邻甲氧基苯胺多聚体纳米结构(POMANS)。使用透射电子显微镜(TEM)表征和傅里叶变换红外光谱(FTIR)来表征POMANS。然后使用这种聚合物来构

尿酸(UA)和叶酸(FA)一直是生物医学所关注的化合物。在人类中,大约70%的每日尿酸通过肾脏产生,5- 25%的人由于肾排泄障碍导致高尿酸血症。众所周知,叶酸属于B族维生素,女性常补充以防止孕期神经管缺陷的发生。

本研究中,研究人员使用新型两步法合成邻甲氧基苯胺多聚体纳米结构(POMANS)。使用透射电子显微镜(TEM)表征和傅里叶变换红外光谱(FTIR)来表征POMANS。然后使用这种聚合物来构建一个改良的多壁碳纳米管、石墨电极(POMANS-MWCNT/GPE)。使用线性扫描伏安法(LSV)、循环伏安法(CV)以及计时电流法来研究邻甲氧基苯胺多聚体与多壁碳纳米管糊电极作为一种调节器在各种PHs水溶液中对UA和FA的电催化氧化的稳定性。

结果显示,在pH=6.0的水溶液中,POMANS-MWCNT/GPE对UA和FA的电氧化具有较高的阳极峰值电流。在优化条件下获得了催化峰电流,与UA和FA的浓度呈线性相关,分别在0.6-52和0.5-68μM范围内,UA与FA的检测极限分别为0.157和0.113μM。

总之,该研究结果表明,研究人员成功开发了一种灵敏、简便、廉价的电化学传感器,可用于尿样和血清等样品中UA和FA的同时测定。

原始出处:

Hossein Rajabi, Meissam Noroozifar. New synthesis of poly ortho-methoxyaniline nanostructures and its application to construct modified multi-wall carbon nanotube/graphite paste electrode for simultaneous determination of uric acid and folic acid. Mater Sci Eng C Mater Biol Appl. 2017 Jun 1; 75: 791–797. doi: 10.1016/j.msec.2017.02.133.

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    2018-07-13 kksonne
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    2017-08-12 mjldent
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    2017-08-10 情途末路

    感谢分享,希望能给临床工作带来便利

    0

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    2017-08-10 131****1460

    学习了受益匪浅。

    0

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    2017-08-10 xiaotaiyang1

    学习学习下,谢谢分享

    0

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    2017-08-10 1771ae4158m

    学习一下很不错

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    2017-08-10 1551f3efb1m

    高端...

    0

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